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  Evolution of copper resistance in the kiwifruit pathogen Pseudomonas syringae pv. actinidiae through acquisition of integrative conjugative elements and plasmids

Colombi, E., Straub, C., Künzel, S., Templeton, M. D., McCann, H. C., & Rainey, P. B. (2017). Evolution of copper resistance in the kiwifruit pathogen Pseudomonas syringae pv. actinidiae through acquisition of integrative conjugative elements and plasmids. Environmental Microbiology, 19(2), 819-832. doi:10.1111/1462-2920.13662.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-9034-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-1D08-3
Genre: Journal Article

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 Creators:
Colombi, Elena1, Author
Straub, Christina, Author
Künzel, Sven1, Author              
Templeton, Matthew D., Author
McCann, Honour C., Author
Rainey, Paul B.2, Author              
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
2Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2421699              

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 Abstract: Horizontal gene transfer can precipitate rapid evolutionary change. In 2010 the global pandemic of kiwifruit canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) reached New Zealand. At the time of introduction, the single clone responsible for the outbreak was sensitive to copper, however, analysis of a sample of isolates taken in 2015 and 2016 showed that a quarter were copper resistant. Genome sequences of seven strains showed that copper resistance – comprising czc/cusABC and copABCD systems – along with resistance to arsenic and cadmium, was acquired via uptake of integrative conjugative elements (ICEs), but also plasmids. Comparative analysis showed ICEs to have a mosaic structure, with one being a tripartite arrangement of two different ICEs and a plasmid that were isolated in 1921 (USA), 1968 (NZ) and 1988 (Japan), from P. syringae pathogens of millet, wheat and kiwifruit respectively. Two of the Psa ICEs were nearly identical to two ICEs isolated from kiwifruit leaf colonists prior to the introduction of Psa into NZ. Additionally, we show ICE transfer in vitro and in planta, analyze fitness consequences of ICE carriage, capture the de novo formation of novel recombinant ICEs, and explore ICE host-range.

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Language(s): eng - English
 Dates: 2016-08-182017-01-022017-02-012017-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1111/1462-2920.13662
 Degree: -

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Title: Environmental Microbiology
  Other : Environmental Microbiology and Environmental Microbiology Reports
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Science
Pages: - Volume / Issue: 19 (2) Sequence Number: - Start / End Page: 819 - 832 Identifier: ISSN: 1462-2912
CoNE: /journals/resource/959328105031