English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A mutually exclusive stem-loop arrangement in roX2 RNA is essential for X-chromosome regulation in Drosophila

Ilik, I. A., Maticzka, D., Georgiev, P., Gutierrez, N. M., Backofen, R., & Akhtar, A. (2017). A mutually exclusive stem-loop arrangement in roX2 RNA is essential for X-chromosome regulation in Drosophila. Genes and Development, 31, 1973-1987. doi:10.1101/gad.304600.117.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Ilik et al..pdf (Publisher version), 12MB
 
File Permalink:
-
Name:
Ilik et al..pdf
Description:
-
Visibility:
Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Ilik, Ibrahim Avsar1, Author              
Maticzka, David2, Author
Georgiev, Plamen1, Author              
Gutierrez, Noel Marie1, Author
Backofen, Rolf2, Author
Akhtar, Asifa1, Author              
Affiliations:
1Department of Chromatin Regulation, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243643              
2Bioinformatics Group, University of Freiburg, Freiburg, Germany, ou_persistent22              

Content

show
hide
Free keywords: dosage compensation, H4K16ac, MLE, X chromosome, acetylation, roX RNA
 Abstract: The X chromosome provides an ideal model system to study the contribution of RNA-protein interactions in epigenetic regulation. In male flies, roX long noncoding RNAs (lncRNAs) harbor several redundant domains to interact with the ubiquitin ligase male-specific lethal 2 (MSL2) and the RNA helicase Maleless (MLE) for X-chromosomal regulation. However, how these interactions provide the mechanics of spreading remains unknown. By using the uvCLAP (UV cross-linking and affinity purification) methodology, which provides unprecedented information about RNA secondary structures in vivo, we identified the minimal functional unit of roX2 RNA. By using wild-type and various MLE mutant derivatives, including a catalytically inactive MLE derivative, MLEGET, we show that the minimal roX RNA contains two mutually exclusive stem-loops that exist in a peculiar structural arrangement: When one stem-loop is unwound by MLE, an alternate structure can form, likely trapping MLE in this perpetually structured region. We show that this functional unit is necessary for dosage compensation, as mutations that disrupt this formation lead to male lethality. Thus, we propose that roX2 lncRNA contains an MLE-dependent affinity switch to enable reversible interactions of the MSL complex to allow dosage compensation of the X chromosome.

Details

show
hide
Language(s): eng - English
 Dates: 2017-10-01
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1101/gad.304600.117
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genes and Development
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cold Spring Harbor Laboratory Press
Pages: - Volume / Issue: 31 Sequence Number: - Start / End Page: 1973 - 1987 Identifier: ISSN: 0890-9369
CoNE: https://pure.mpg.de/cone/journals/resource/954925557453