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  The structure of the COPI coat determined within the cell.

Bykov, Y. S., Schaffer, M., Dodonova, S. O., Albert, S., Plitzko, J. M., Baumeister, W., et al. (2017). The structure of the COPI coat determined within the cell. eLife, 6: e32493. doi:10.7554/eLife.32493.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-9164-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-9167-B
Genre: Journal Article

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Bykov, Y. S., Author
Schaffer, M., Author
Dodonova, S. O.1, Author              
Albert, S., Author
Plitzko, J. M., Author
Baumeister, W., Author
Engel, B. D., Author
Briggs, J. A. G., Author
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively studied with in vitro reconstitution systems using purified components. Previously we have determined a complete structural model of the in vitro reconstituted COPI coat (Dodonova et al., 2017). Here, we applied cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determine the native structure of the COPI coat within vitrified Chlamydomonas reinhardtii cells. The native algal structure resembles the in vitro mammalian structure, but additionally reveals cargo bound beneath beta'-COP. We find that all coat components disassemble simultaneously and relatively rapidly after budding. Structural analysis in situ, maintaining Golgi topology, shows that vesicles change their size, membrane thickness, and cargo content as they progress from cis to trans, but the structure of the coat machinery remains constant.

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Language(s): eng - English
 Dates: 2017-11-17
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.32493
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Title: eLife
Source Genre: Journal
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Pages: 18 Volume / Issue: 6 Sequence Number: e32493 Start / End Page: - Identifier: -