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  Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa.

Sonnleitner, E., Wulf, A., Campagne, S., Pei, X. Y., Wolfinger, M. T., Forlani, G., et al. (2018). Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa. Nucleic Acids Research, 46(3), 1470-1485. doi:10.1093/nar/gkx1245.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-98EA-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-B9C8-B
Genre: Journal Article

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Sonnleitner, E., Author
Wulf, A.1, Author              
Campagne, S., Author
Pei, X. Y., Author
Wolfinger, M. T., Author
Forlani, G., Author
Prindl, K., Author
Abdou, L., Author
Resch, A., Author
Allain, F. H., Author
Luisi, B. F., Author
Urlaub, H.1, Author              
Bläsi, U., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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 Abstract: In Pseudomonas aeruginosa the RNA chaperone Hfq and the catabolite repression control protein (Crc) act as post-transcriptional regulators during carbon catabolite repression (CCR). In this regard Crc is required for full-fledged Hfq-mediated translational repression of catabolic genes. RNAseq based transcriptome analyses revealed a significant overlap between the Crc and Hfq regulons, which in conjunction with genetic data supported a concerted action of both proteins. Biochemical and biophysical approaches further suggest that Crc and Hfq form an assembly in the presence of RNAs containing A-rich motifs, and that Crc interacts with both, Hfq and RNA. Through these interactions, Crc enhances the stability of Hfq/Crc/RNA complexes, which can explain its facilitating role in Hfq-mediated translational repression. Hence, these studies revealed for the first time insights into how an interacting protein can modulate Hfq function. Moreover, Crc is shown to interfere with binding of a regulatory RNA to Hfq, which bears implications for riboregulation. These results are discussed in terms of a working model, wherein Crc prioritizes the function of Hfq toward utilization of favored carbon sources.

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Language(s): eng - English
 Dates: 2017-12-132018-02-16
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/nar/gkx1245
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 46 (3) Sequence Number: - Start / End Page: 1470 - 1485 Identifier: -