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  Actin Waves do not boost neurite outgrowth in the early stages of neuron maturation.

Mortal, S., Iseppon, F., Perissinotto, A., D'Este, E., Cojoc, D., Napolitano, L. M. R., et al. (2017). Actin Waves do not boost neurite outgrowth in the early stages of neuron maturation. Frontiers in Cellular Neuroscience, 11: 402. doi:10.3389/fncel.2017.00402.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-9B8D-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-3B9F-B
Genre: Journal Article

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Mortal, S., Author
Iseppon, F., Author
Perissinotto, A., Author
D'Este, E.1, Author              
Cojoc, D., Author
Napolitano, L. M. R., Author
Torre, V., Author
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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Free keywords: actin waves (AWs); growth cones (GCs); Myosin IIB; beta-cyclodextrin; RhoGTPases
 Abstract: During neurite development, Actin Waves (AWs) emerge at the neurite base and move up to its tip, causing a transient retraction of the Growth Cone (GC). Many studies have shown that AWs are linked to outbursts of neurite growth and, therefore, contribute to the fast elongation of the nascent axon. Using long term live cell-imaging, we show that AWs do not boost neurite outgrowth and that neurites without AWs can elongate for several hundred microns. Inhibition of Myosin II abolishes the transient GC retraction and strongly modifies the AWs morphology. Super-resolution nanoscopy shows that Myosin IIB shapes the growth cone-like AWs structure and is differently distributed in AWs and GCs. Interestingly, depletion of membrane cholesterol and inhibition of Rho GTPases decrease AWs frequency and velocity. Our results indicate that Myosin IIB, membrane tension, and small Rho GTPases are important players in the regulation of the AW dynamics. Finally, we suggest a role for AWs in maintaining the GCs active during environmental exploration.

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Language(s): eng - English
 Dates: 2017-12-18
 Publication Status: Published online
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3389/fncel.2017.00402
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Title: Frontiers in Cellular Neuroscience
Source Genre: Journal
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Pages: 15 Volume / Issue: 11 Sequence Number: 402 Start / End Page: - Identifier: -