English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Actin Waves do not boost neurite outgrowth in the early stages of neuron maturation.

Mortal, S., Iseppon, F., Perissinotto, A., D'Este, E., Cojoc, D., Napolitano, L. M. R., et al. (2017). Actin Waves do not boost neurite outgrowth in the early stages of neuron maturation. Frontiers in Cellular Neuroscience, 11: 402. doi:10.3389/fncel.2017.00402.

Item is

Files

show Files
hide Files
:
2518540.pdf (Publisher version), 6MB
Name:
2518540.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Mortal, S., Author
Iseppon, F., Author
Perissinotto, A., Author
D'Este, E.1, Author           
Cojoc, D., Author
Napolitano, L. M. R., Author
Torre, V., Author
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: actin waves (AWs); growth cones (GCs); Myosin IIB; beta-cyclodextrin; RhoGTPases
 Abstract: During neurite development, Actin Waves (AWs) emerge at the neurite base and move up to its tip, causing a transient retraction of the Growth Cone (GC). Many studies have shown that AWs are linked to outbursts of neurite growth and, therefore, contribute to the fast elongation of the nascent axon. Using long term live cell-imaging, we show that AWs do not boost neurite outgrowth and that neurites without AWs can elongate for several hundred microns. Inhibition of Myosin II abolishes the transient GC retraction and strongly modifies the AWs morphology. Super-resolution nanoscopy shows that Myosin IIB shapes the growth cone-like AWs structure and is differently distributed in AWs and GCs. Interestingly, depletion of membrane cholesterol and inhibition of Rho GTPases decrease AWs frequency and velocity. Our results indicate that Myosin IIB, membrane tension, and small Rho GTPases are important players in the regulation of the AW dynamics. Finally, we suggest a role for AWs in maintaining the GCs active during environmental exploration.

Details

show
hide
Language(s): eng - English
 Dates: 2017-12-18
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3389/fncel.2017.00402
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Frontiers in Cellular Neuroscience
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 15 Volume / Issue: 11 Sequence Number: 402 Start / End Page: - Identifier: -