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  Initial contact guidance during cell spreading is contractility-independent

Sales, A., Holle, A. W., & Kemkemer, R. (2017). Initial contact guidance during cell spreading is contractility-independent. Soft Matter, 13, 5158-5167. doi:10.1039/C6SM02685K.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-245A-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-2A9C-2
Genre: Journal Article

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 Creators:
Sales, Adriá, Author              
Holle, Andrew W.1, Author              
Kemkemer, Ralf1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: A wide variety of cell types exhibit substrate topography-based behavior, also known as contact guidance. However, the precise cellular mechanisms underlying this process are still unknown. In this study, we investigated contact guidance by studying the reaction of human endothelial cells (ECs) to well-defined microgroove topographies, both during and after initial cell spreading. As the cytoskeleton plays a major role in cellular adaptation to topographical features, two methods were used to perturb cytoskeletal structures. Inhibition of actomyosin contractility with the chemical inhibitor blebbistatatin demonstrated that initial contact guidance events are independent of traction force generation. However, cell alignment to the grooved substrate was altered at later time points, suggesting an initial ‘passive’ phase of contact guidance, followed by a contractility-dependent ‘active’ phase that relies on mechanosensitive feedback. The actin cytoskeleton was also perturbed in an indirect manner by culturing cells upside down, resulting in decreased levels of contact guidance and suggesting that a possible loss of contact between the actin cytoskeleton and the substrate could lead to cytoskeleton impairment. The process of contact guidance at the microscale was found to be primarily lamellipodia driven, as no bias in filopodia extension was observed on micron-scale grooves.

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Language(s): eng - English
 Dates: 2016-11-302017-06-162017-06-162017
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1039/C6SM02685K
 Degree: -

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Title: Soft Matter
  Abbreviation : Soft Matter
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 13 Sequence Number: - Start / End Page: 5158 - 5167 Identifier: ISSN: 1744-683X
CoNE: https://pure.mpg.de/cone/journals/resource/1744-683X