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  A proximity labeling strategy provides insights into the composition and dynamics of lipid droplet proteomes.

Bersuker, K., Peterson, C. W. H., To, M., Sahl, S. J., Savikhin, V., Grossman, E. A., et al. (2018). A proximity labeling strategy provides insights into the composition and dynamics of lipid droplet proteomes. Developmental Cell, 44(1), 97-112. doi:10.1016/j.devcel.2017.11.020.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-2558-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-33E5-1
Genre: Journal Article

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 Creators:
Bersuker, K., Author
Peterson, C. W. H., Author
To, M., Author
Sahl, S. J.1, Author              
Savikhin, V., Author
Grossman, E. A., Author
Nomura, D. K., Author
Olzmann, J. A., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: APEX; APEX2; ERAD; biotinylation; endoplasmic reticulum; lipid droplet; proteasome; proteome; proximity labeling; ubiquitin
 Abstract: Lipid droplet (LD) functions are regulated by a complement of integral and peripheral proteins that associate with the bounding LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to two different cell types identified the vast majority of previously validated LD proteins, excluded common contaminating proteins, and revealed new LD proteins. Moreover, quantitative analysis of LD proteome dynamics uncovered a role for endoplasmic reticulum-associated degradation in controlling the composition of the LD proteome. These data provide an important resource for future LD studies and demonstrate the utility of proximity labeling to study the regulation of LD proteomes.

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Language(s): eng - English
 Dates: 2017-12-212018-01-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.devcel.2017.11.020
 Degree: -

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Title: Developmental Cell
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 44 (1) Sequence Number: - Start / End Page: 97 - 112 Identifier: -