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  Crystallization and preliminary characterization of chloromuconolactone dehalogenase from Rhodococcus opacus 1CP

Roth, C., Kaschabek, S. R., Gröning, J. A. D., Handrek, T., Schlömann, M., & Sträter, N. (2012). Crystallization and preliminary characterization of chloromuconolactone dehalogenase from Rhodococcus opacus 1CP. Acta Crystallographica Section F, 68(5), 591-595. doi:10.1107/S1744309112011888.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-2555-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-2556-6
Genre: Journal Article

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 Creators:
Roth, Christian1, Author              
Kaschabek, Stefan R., Author
Gröning, Janosch A. D., Author
Handrek, Thomas, Author
Schlömann, Michael, Author
Sträter, Norbert, Author
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1External Organizations, ou_persistent22              

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Free keywords: chloromuconolactone dehalogenase, Rhodococcus opacus 1CP
 Abstract: Chloroaromatic compounds are often very persistent environmental pollutants. Nevertheless, numerous bacteria are able to metabolize these compounds and to utilize them as sole energy and carbon sources. ıt Rhodococcus opacus} 1CP is able to degrade several chloroaromatic compounds, some of them {ıt via} a variation of the 3-chlorocatechol branch of the modified {ıt ortho}-cleavage pathway. This branch in {ıt R. opacus} differs from that in {ıt Proteobacteria} in the inability of the chloromuconate cycloisomerase to dehalogenate. Instead, a unique enzyme designated as chloromuconolactone dehalogenase (ClcF) is recruited. ClcF dehalogenates 5-chloromuconolactone to {ıt cis}-dienelactone and shows a high similarity to muconolactone isomerases (EC 5.3.3.4). However, unlike the latter enzymes, it is unable to catalyse the isomerization of muconolactone to 3-⁻oxoadipate enollactone. In order to characterize the catalytic mechanism of this unusual dehalogenase, the enzyme was crystallized and subjected to X-ray structural analysis. Data sets to up to 1.65Å resolution were collected from two different crystal forms using synchrotron radiation. Crystal form I (space group {ıt P}2{\sb 1}) contained 40 subunits in the asymmetric unit, whereas ten subunits were present in crystal form II (space group {ıt P}2{\sb 1}2{\sb 1}2{\sb 1). The self-rotation function revealed the orientations of the molecular symmetry axes of the homodecamer of 52 symmetry.

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 Dates: 2012-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1107/S1744309112011888
BibTex Citekey: Roth:ub5030
 Degree: -

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Title: Acta Crystallographica Section F
Source Genre: Journal
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Publ. Info: Blackwell Publishing Limited
Pages: - Volume / Issue: 68 (5) Sequence Number: - Start / End Page: 591 - 595 Identifier: ISSN: 1744-3091