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  Cell-accurate optical mapping across the entire developing heart

Weber, M., Scherf, N., Meyer, A. M., Panáková, D., Kohl, P., & Huisken, J. (2017). Cell-accurate optical mapping across the entire developing heart. eLife, 6: e28307. doi:10.7554/eLife.28307.001.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-27A9-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-4FC2-8
Genre: Journal Article

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 Creators:
Weber, Michael1, 2, Author
Scherf, Nico1, 3, Author              
Meyer, Alexander M.4, Author
Panáková, Daniela4, 5, Author
Kohl, Peter6, Author
Huisken, Jan1, 7, Author
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, ou_persistent22              
2Harvard Medical School, Boston, MA, USA, ou_persistent22              
3Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              
4Max Delbrück Center for Molecular Medicine, Berlin, Germany, ou_persistent22              
5German Centre for Cardiovascular Research, Berlin, Germany, ou_persistent22              
6Faculty of Medicine, Albert Ludwigs University Freiburg, Germany, ou_persistent22              
7Morgridge Institute for Research, Madison, WI, USA, ou_persistent22              

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 Abstract: Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca2+-mapping of all cells in the entire electro-mechanically uncoupled heart during the looping stage of live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs.

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Language(s): eng - English
 Dates: 2017-12-29
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.7554/eLife.28307.001
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Project name : Cardiac connective tissue: Beat-by-beat relevance for heart function in health and disease / CARDIONECT
Grant ID : 323099
Funding program : Funding Programme 7
Funding organization : European Commission (EC)
Project name : -
Grant ID : VH-NG-736
Funding program : Young Investigator Program
Funding organization : Helmholtz-Gemeinschaft

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 6 Sequence Number: e28307 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X