English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  X-ray damage to the Mn4Ca complex in single crystals of photosystem II: A case study for metalloprotein crystallography

Yano, J., Kern, J., Irrgang, K., Latimer, M. J., Bergmann, U., Glatzel, P., et al. (2005). X-ray damage to the Mn4Ca complex in single crystals of photosystem II: A case study for metalloprotein crystallography. Proceedings of the National Academy of Sciences of the USA, 102(34), 12047-12052. doi:10.1073/pnas.0505207102.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0000-3841-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-3842-7
Genre: Journal Article

Files

show Files
hide Files
:
PNAS_102_2005_12047.pdf (Any fulltext), 548KB
 
File Permalink:
-
Name:
PNAS_102_2005_12047.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Locator:
https://doi.org/10.1073/pnas.0505207102 (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Yano, Junko, Author
Kern, Jana1, 2, Author              
Irrgang, Klaus−Dieter, Author
Latimer, Matthew J., Author
Bergmann, Uwe, Author
Glatzel, Pieter, Author
Pushkar, Yulia N., Author
Biesiadka, Jacek, Author
Loll, Bernhard3, Author              
Sauer, Kenneth, Author
Messinger, Johannes, Author
Zouni, Athina, Author
Yachandra, Vittal K., Author
Affiliations:
1Structure of neocortical circuits, Max Planck Institute for Medical Research, Max Planck Society, ou_1497742              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
3Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: manganese, oxygen evolution, water oxidation, x-ray spectroscopy
 Abstract: X-ray absorption spectroscopy was used to measure the damage caused by exposure to x-rays to the Mn(4)Ca active site in single crystals of photosystem II as a function of dose and energy of x-rays, temperature, and time. These studies reveal that the conditions used for structure determination by x-ray crystallography cause serious damage specifically to the metal-site structure. The x-ray absorption spectra show that the structure changes from one that is characteristic of a high-valent Mn(4)(III(2),IV(2)) oxo-bridged Mn(4)Ca cluster to that of Mn(II) in aqueous solution. This damage to the metal site occurs at a dose that is more than one order of magnitude lower than the dose that results in loss of diffractivity and is commonly considered safe for protein crystallography. These results establish quantitative x-ray dose parameters that are applicable to redox-active metalloproteins. This case study shows that a careful evaluation of the structural intactness of the active site(s) by spectroscopic techniques can validate structures derived from crystallography and that it can be a valuable complementary method before structure-function correlations of metalloproteins can be made on the basis of high-resolution x-ray crystal structures.

Details

show
hide
Language(s): eng - English
 Dates: 2005-04-242005-08-23
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences of the USA
  Alternative Title : PNAS
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 102 (34) Sequence Number: - Start / End Page: 12047 - 12052 Identifier: -