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  Thermostability and Ca2+ Binding Properties of Wild Type and Heterologously Expressed PsbO Protein from Cyanobacterial Photosystem II

Loll, B., Gerold, G., Slowik, D., Voelter, W. J., Jung, C., Saenger, W., et al. (2005). Thermostability and Ca2+ Binding Properties of Wild Type and Heterologously Expressed PsbO Protein from Cyanobacterial Photosystem II. Biochemistry, 44(12), 4691-4698. doi:10.1021/bi047614r.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-3973-F Version Permalink: http://hdl.handle.net/21.11116/0000-0000-3974-E
Genre: Journal Article

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 Creators:
Loll, Bernhard1, Author              
Gerold, Gisa, Author
Slowik, Daria, Author
Voelter, Wolfgang J., Author
Jung, Christiane, Author
Saenger, Wolfram, Author
Irrgang, Klaus−Dieter, Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Oxygenic photosynthesis takes place in the thylakoid membrane of cyanobacteria, algae, and higher plants. Initially light is absorbed by an oligomeric pigment-protein complex designated as photosystem II (PSII), which catalyzes light-induced water cleavage under release of molecular oxygen for the biosphere on our planet. The membrane-extrinsic manganese stabilizing protein (PsbO) is associated on the lumenal side of the thylakoids close to the redox-active (Mn)(4)Ca cluster at the catalytically active site of PSII. Recombinant PsbO from the thermophilic cyanobacterium Thermosynechococcus elongatus was expressed in Escherichia coli and spectroscopically characterized. The secondary structure of recombinant PsbO (recPsbO) was analyzed in the absence and presence of Ca(2+) using Fourier transform infrared spectroscopy (FTIR) and circular dichroism spectropolarimetry (CD). No significant structural changes could be observed when the PSII subunit was titrated with Ca(2+) in vitro. These findings are compared with data for spinach PsbO. Our results are discussed in the light of the recent 3D-structural analysis of the oxygen-evolving PSII and structural/thermodynamic differences between the two homologous proteins from thermophilic cyanobacteria and plants

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Language(s): eng - English
 Dates: 2005-03-212004-11-112005-03-052005-03-052005-03-29
 Publication Status: Published in print
 Pages: 8
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 Rev. Type: Peer
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Title: Biochemistry
  Alternative Title : Biochem.
Source Genre: Journal
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Pages: - Volume / Issue: 44 (12) Sequence Number: - Start / End Page: 4691 - 4698 Identifier: -