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  Bacterial expression, purification and preliminary X-ray crystallographic characterization of the invertase inhibitor Nt-CIF from tobacco

Hothorn, M., Bonneau, F., Stier, G., Greinerb, S., & Scheffzek, K. (2003). Bacterial expression, purification and preliminary X-ray crystallographic characterization of the invertase inhibitor Nt-CIF from tobacco. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen), 59(12), 2279-2282. doi:10.1107/S0907444903021036.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-3B65-D Version Permalink: http://hdl.handle.net/21.11116/0000-0000-3B6B-7
Genre: Journal Article

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ActaCrystD_59_2003_2279.pdf (Any fulltext), 322KB
 
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 Creators:
Hothorn, Michael, Author
Bonneau, Fabien, Author
Stier, Gunter1, Author              
Greinerb, Steffen, Author
Scheffzek, Klaus2, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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Free keywords: invertases; pectin methylesterase; post-translational regulation; plant enzymes; carbohydrate metabolism; Nicotiana tabacum
 Abstract: Acid invertases catalyzing the breakdown of sucrose are regulated at the post-translational level by extracellular inhibitory proteins of 16-20 kDa molecular weight in a pH-dependent manner. Little is known about the characteristics of the underlying protein-protein interaction. Here, the expression, purification, characterization, crystallization and initial X-ray analysis of a biologically active invertase inhibitor Nt-CIF from tobacco is reported. Four crystal forms covering a wide pH range have been obtained and data sets at resolutions higher than 2.5 A have been collected.

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Language(s): eng - English
 Dates: 2003-07-232003-09-232003-12
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Acta Crystallographica. Section D: Biological Crystallography (Copenhagen)
  Other : Acta Crystallogr D
Source Genre: Journal
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Publ. Info: [Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard]
Pages: - Volume / Issue: 59 (12) Sequence Number: - Start / End Page: 2279 - 2282 Identifier: ISSN: 0907-4449
CoNE: https://pure.mpg.de/cone/journals/resource/954925562619