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  Crystallization and preliminary X-ray diffraction analysis of monoprenylated Rab7 GTPase in complex with Rab escort protein 1

Rak, A., Pylypenko, O., Niculae, A., Goody, R. S., & Alexandrov, K. (2003). Crystallization and preliminary X-ray diffraction analysis of monoprenylated Rab7 GTPase in complex with Rab escort protein 1. Journal of Structural Biology, 141(1), 93-95. doi:10.1016/S1047-8477(02)00634-2.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-3DA6-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-3DA7-0
Genre: Journal Article

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 Creators:
Rak, Alexey, Author
Pylypenko, Olena1, Author              
Niculae, Anca, Author
Goody, Roger S.2, Author              
Alexandrov, Kirill, Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort protein (REP). REP functions as a molecular chaperone that presents Rab proteins to the RabGGTase and after prenylation delivers them to their target membrane. Mutations in the REP-1 gene in humans lead to an X-chromosome-linked defect known as choroideremia, a progressive disease that inevitably culminates in complete blindness. Here we report in vitro assembly, purification, and crystallization of the monoprenylated Rab7GDP:REP-1 complex. X-Ray diffraction data for the REP-1:Rab7 complex were collected to 2.2-A resolution at the ESRF. The crystals belong to the orthorhombic space group P2(1)2(1)2 with unit-cell parameters a=64.3A, b=105.3A, c=132.6A. Preliminary structural analysis revealed the presence of one complex in the asymmetric unit. To understand the conformational changes in Rab protein on complex formation we also crystallized the GDP-bound form of Rab7 that diffracted to at least 1.8A on the in-house X-ray source.

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Language(s): eng - English
 Dates: 2002-07-302002-11-272003-01-152003-01-01
 Publication Status: Published in print
 Pages: 3
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 Table of Contents: -
 Rev. Type: Peer
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Title: Journal of Structural Biology
  Abbreviation : J. Struct. Biol.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 141 (1) Sequence Number: - Start / End Page: 93 - 95 Identifier: ISSN: 1047-8477
CoNE: https://pure.mpg.de/cone/journals/resource/954922650160