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  Long-term, noninvasive imaging of regulated gene expression in living mice

Hasan, M. T., Schönig, K., Berger, S., Graewe, W., & Bujard, H. (2001). Long-term, noninvasive imaging of regulated gene expression in living mice. Genesis, 29(3), 116-122. doi:10.1002/gene.1014.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-3F00-A Version Permalink: http://hdl.handle.net/21.11116/0000-0000-3F01-9
Genre: Journal Article

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Genesis_29_2001_116.pdf (Any fulltext), 571KB
 
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 Creators:
Hasan, Mazahir T.1, 2, 3, Author              
Schönig, Kai, Author
Berger, Stefan, Author
Graewe, Walter, Author
Bujard, Hermann, Author
Affiliations:
1Mazahir Hasan Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497726              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
3Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: tetracycline; regulatable transcription; Cre recombinase; firefly luciferase; bioluminescence; transgenics
 Abstract: We describe here an approach for monitoring regulated gene expression by noninvasive imaging in living mice. We have utilized the tetracycline inducible system to simultaneously coregulate the expression of two genes encoding the firefly luciferase and the Cre recombinase, respectively. Results from our model system demonstrate that luciferase can be used as a noninvasive imaging marker for the regulated expression of a second gene in living mice. The integration of noninvasive imaging and inducible gene expression into current approaches of functional genomics should greatly advance our capabilities of carrying out highly controlled long-term studies of gene function in individual mice.

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Language(s): eng - English
 Dates: 2000-07-172000-12-302001-03-082001-03
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Genesis
Source Genre: Journal
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Publ. Info: New York, NY : Wiley-Liss
Pages: - Volume / Issue: 29 (3) Sequence Number: - Start / End Page: 116 - 122 Identifier: ISSN: 1526-954X
CoNE: https://pure.mpg.de/cone/journals/resource/954925489840