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  Depolarization, intracellular calcium and exocytosis in single vertebrate nerve endings

Lindau, M., Stuenkel, E. L., & Nordmann, J. J. (1992). Depolarization, intracellular calcium and exocytosis in single vertebrate nerve endings. Biophysical Journal, 61(1), 19-30. doi:10.1016/S0006-3495(92)81812-X.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-606F-8 Version Permalink: https://hdl.handle.net/21.11116/0000-0000-6070-5
Genre: Journal Article

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BiophysJ_61_1992_19.pdf (Any fulltext), 5MB
 
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Lindau, Manfred1, Author           
Stuenkel, Edward L., Author
Nordmann, Jean J., Author
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1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: We have investigated the temporal relationship between depolarization, elevation of [Ca2+]i and exocytosis in single vertebrate neuroendocrine nerve terminals. The change of [Ca2+]i and vasopressin release were measured with a time resolution of less than 1 s in response to K(+)-induced depolarization. Exocytosis was also monitored in the whole-terminal patch-clamp configuration by time resolved capacitance measurements while [Ca2+]i was simultaneously followed by fura-2 fluorescence measurements. In intact as well as patch-clamped nerve terminals sustained depolarization leads to a sustained rise of [Ca2+]i. The rate of vasopressin release from intact nerve terminals rises in parallel with [Ca2+]i but then declines rapidly towards basal (t1/2 approximately 15 s) despite the maintained high [Ca2+]i indicating that only a limited number of exocytotic vesicles can be released. We demonstrate that in nerve terminals exocytosis can be followed during step depolarization by capacitance measurements. The capacitance increase starts instantaneously whereas [Ca2+]i rises with a half time of several hundred milliseconds. An instantaneous steep capacitance increase is followed by a slow increase with a slope of 25-50 fF/s indicating the sequential fusion of predocked and cytoplasmic vesicles. During depolarization the capacitance slope declines to zero with a similar time course as the vasopressin release indicating a decrease in exocytotic activity. Depolarization per se in the absence of a sufficient rise of [Ca2+]i does not induce exocytosis but elevation of [Ca2+]i in the absence of depolarization is as effective as in its presence. The experiments suggest that a rapid rise of [Ca2+]i in a narrow region beneath the plasma membrane induces a burst of exocytotic activity preceding the elevation of bulk [Ca2+]i in the whole nerve terminal.

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Language(s): eng - English
 Dates: 1991-05-011991-08-222009-01-061992-01
 Publication Status: Issued
 Pages: 12
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0006-3495(92)81812-X
URI: https://www.ncbi.nlm.nih.gov/pubmed/1540689
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 61 (1) Sequence Number: - Start / End Page: 19 - 30 Identifier: Other: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117