ausblenden:
Schlagwörter:
-
Zusammenfassung:
Background:
Two cembranoid diterpenes, [(1S, 2E, 4R, 6R, 7E, 11E)-2, 7, 11-cembratriene-4, 6-diol] (1) and its epimer [(1S, 2E, 4S,
6R,7E,11E)-2, 7, 11-cembratriene-4, 6-diol] (2) were isolated from surface dichloromethane washings and chloroform extract of
Nicotiana sylvestris leaves.
Methods:
The compounds were purified using silica gel column- thin layer- and flash column chromatography methods. The structures of the
isolated compounds were elucidated using spectroscopic analysis and their 1H and 13C NMR spectroscopic data were compared with
those of authentic samples reported in the literature. The cytotoxic activity of 1 and 2 against three human cancer cell lines, including
LS180 (human colon adenocarcinoma), MCF-7 (human breast adenocarcinoma) and MOLT-4 (human lymphoblastic leukemia) were
evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric bioassay.
