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  Amino-terminal leucine-rich repeats in gonadotropin receptors determine hormone selectivity

Braun, T., Schofield, P. R., & Sprengel, R. (1991). Amino-terminal leucine-rich repeats in gonadotropin receptors determine hormone selectivity. The EMBO Journal, 10(7), 1885-1890. doi:10.1002/j.1460-2075.1991.tb07714.x.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-641C-1 Version Permalink: https://hdl.handle.net/21.11116/0000-0000-641D-0
Genre: Journal Article

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 Creators:
Braun, Thomas1, Author           
Schofield, Peter R., Author
Sprengel, Rolf2, 3, 4, Author           
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
3Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              
4Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society, ou_1497733              

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 Abstract: Recombinant expression of truncated receptors for luteinizing hormone/chorionic gonadotropin (LH/CG) revealed that the amino-terminal leucine-rich repeats 1-8 of the extracellular receptor domain bind human chorionic gonadotropin (hCG) with an affinity (Kd = 0.72 +/- 0.2 nM) similar to that of the native LH/CG receptor (Kd = 0.48 +/- 0.05 nM). LH/CG receptor leucine-rich repeats 1-8 were used to replace homologous sequences in the closely related receptor for follicle stimulating hormone (FSH). Cells expressing such chimeric LH/CG-FSH receptors bind hCG and show elevated cylic AMP levels when stimulated by hCG but not by recombinant human FSH (rhFSH). Similarly, a chimeric LH/CG receptor in which leucine-rich repeats 1-11 originated from the FSH receptor is activated by rhFSH but not by hCG. For this chimera, no residual [125I] hCG binding was observed in a range of 2 pM to 10 nM. Our results demonstrate that specificity of gonadotropin receptors is determined by a high affinity hormone binding site formed by the amino-terminal leucine-rich receptor repeats.

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Language(s): eng - English
 Dates: 1991-02-141991-03-141991-07-011991
 Publication Status: Issued
 Pages: 6
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 Rev. Type: Peer
 Identifiers: DOI: 10.1002/j.1460-2075.1991.tb07714.x
URI: https://www.ncbi.nlm.nih.gov/pubmed/2050124
URI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC452863/
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Title: The EMBO Journal
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 10 (7) Sequence Number: - Start / End Page: 1885 - 1890 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061_1