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  Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT

Schueder, F., Lara-Gutierrez, J., Beliveau, B. J., Saka, S. K., Sasaki, H. M., Woehrstein, J. B., et al. (2017). Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT. Nature Communications, 8: 2090. doi:10.1038/s41467-017-02028-8.

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 Creators:
Schueder, Florian1, Author              
Lara-Gutierrez, Juanita2, Author
Beliveau, Brian J.2, Author
Saka, Sinem K.2, Author
Sasaki, Hiroshi M.2, Author
Woehrstein, Johannes B.1, Author              
Strauss, Maximilian T.1, Author              
Grabmayr, Heinrich1, Author              
Yin, Peng2, Author
Jungmann, Ralf2, Author
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2external, ou_persistent22              

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Free keywords: OPTICAL RECONSTRUCTION MICROSCOPY; LOCALIZATION MICROSCOPY; FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; EXCHANGE-PAINT; X-CHROMOSOME; PROBES; BINDING; PRECISION; SHAPESScience & Technology - Other Topics;
 Abstract: Single-molecule localization microscopy (SMLM) can visualize biological targets on the nanoscale, but complex hardware is required to perform SMLM in thick samples. Here, we combine 3D DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) with spinning disk confocal (SDC) hardware to overcome this limitation. We assay our achievable resolution with two-and three-dimensional DNA origami structures and demonstrate the general applicability by imaging a large variety of cellular targets including proteins, DNA and RNA deep in cells. We achieve multiplexed 3D super-resolution imaging at sample depths up to similar to 10 mu m with up to 20 nm planar and 80 nm axial resolution, now enabling DNA-based super-resolution microscopy in whole cells using standard instrumentation.

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Language(s): eng - English
 Dates: 2017-12-12
 Publication Status: Published online
 Pages: 9
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 Table of Contents: -
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 Degree: -

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Project name : Emmy Noether Program (DFG JU 2957/1-1), SFB 1032 (Nanoagents for the spatiotemporal control of molecular and cellular reactions), ERC through an ERC Starting Grant (MolMap, Grant agreement number 680241)
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 2090 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723