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  Preparation of A Spaceflight: Apoptosis Search in Sutured Wound Healing Models

Riwaldt, S., Monici, M., Petersen, A. G., Jensen, U. B., Evert, K., Pantalone, D., et al. (2017). Preparation of A Spaceflight: Apoptosis Search in Sutured Wound Healing Models. International Journal of Molecular Sciences, 18(12): 2604. doi:10.3390/ijms18122604.

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ijms-18-02604-v2.pdf (Publisher version), 13MB
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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 Creators:
Riwaldt, Stefan1, Author
Monici, Monica1, Author
Petersen, Asbjorn Graver1, Author
Jensen, Uffe Birk1, Author
Evert, Katja1, Author
Pantalone, Desire1, Author
Utpatel, Kirsten1, Author
Evert, Matthias1, Author
Wehland, Markus1, Author
Krueger, Marcus1, Author
Kopp, Sascha1, Author
Frandsen, Sofie1, Author
Corydon, Thomas1, Author
Sahana, Jayashree1, Author
Bauer, Johann2, Author              
Luetzenberg, Ronald1, Author
Infanger, Manfred1, Author
Grimm, Daniela1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: NF-KAPPA-B; THYROID-CANCER CELLS; EXTRACELLULAR-MATRIX; SIMULATED WEIGHTLESSNESS; ENDOTHELIAL-CELLS; DEATH RECEPTORS; ALTERED GRAVITY; ORGAN-CULTURE; PROTEINS; GROWTHBiochemistry & Molecular Biology; Chemistry; skin; wound healing; suture; apoptosis; caspases; extracellular matrix proteins;
 Abstract: To prepare the ESA (European Space Agency) spaceflight project "Wound healing and Sutures in Unloading Conditions", we studied mechanisms of apoptosis in wound healing models based on ex vivo skin tissue cultures, kept for 10 days alive in serum-free DMEM/F12 medium supplemented with bovine serum albumin, hydrocortisone, insulin, ascorbic acid and antibiotics at 32 degrees C. The overall goal is to test: (i) the viability of tissue specimens; (ii) the gene expression of activators and inhibitors of apoptosis and extracellular matrix components in wound and suture models; and (iii) to design analytical protocols for future tissue specimens after post-spaceflight download. Hematoxylin-Eosin and Elastica-van-Gieson staining showed a normal skin histology with no signs of necrosis in controls and showed a normal wound suture. TdT-mediated dUTP-biotin nick end labeling for detecting DNA fragmentation revealed no significant apoptosis. No activation of caspase-3 protein was detectable. FASL, FADD, CASP3, CASP8, CASP10, BAX, BCL2, CYC1, APAF1, LAMA3 and SPP1 mRNAs were not altered in epidermis and dermis samples with and without a wound compared to 0 day samples (specimens investigated directly post-surgery). BIRC5, CASP9, and FN1 mRNAs were downregulated in epidermis/dermis samples with and/or without a wound compared to 0 day samples. BIRC2, BIRC3 were upregulated in 10 day wound samples compared to 0 day samples in epidermis/dermis. RELA/FAS mRNAs were elevated in 10 day wound and no wound samples compared to 0 day samples in dermis. In conclusion, we demonstrate that it is possible to maintain live skin tissue cultures for 10 days. The viability analysis showed no significant signs of cell death in wound and suture models. The gene expression analysis demonstrated the interplay of activators and inhibitors of apoptosis and extracellular matrix components, thereby describing important features in ex vivo sutured wound healing models. Collectively, the performed methods defining analytical protocols proved to be applicable for post-flight analyzes of tissue specimens after sample return.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published online
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000418896700108
DOI: 10.3390/ijms18122604
 Degree: -

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Title: International Journal of Molecular Sciences
  Abbreviation : Int. J. Mol. Sci.
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 18 (12) Sequence Number: 2604 Start / End Page: - Identifier: ISSN: 1422-0067
CoNE: https://pure.mpg.de/cone/journals/resource/1422-0067