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  Spatiotemporal Control of TGF-β Signaling with Light

Li, Y., Lee, M., Kim, N., Wu, G., Deng, D., Kim, J. M., et al. (2018). Spatiotemporal Control of TGF-β Signaling with Light. ACS Synthetic Biology, 7(2), 443-451. doi:10.1021/acssynbio.7b00225.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-7698-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-EE20-E
Genre: Journal Article

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Copyright © 2017 American Chemical Society
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 Creators:
Li, Yuchao1, Author              
Lee, Minji , Author
Kim, Nury, Author
Wu, Guoyu1, Author              
Deng, Difan1, Author              
Kim, Jin Man , Author
Liu, Xuedong , Author
Heo, Won Do , Author
Zi, Zhike1, Author              
Affiliations:
1Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284              

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Free keywords: Smad2; TGF-beta; cell signaling; optogenetics
 Abstract: Cells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-β) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-β signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-β signaling with high spatiotemporal resolution. Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space. Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations. By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations. The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.

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Language(s): eng - English
 Dates: 2018-01-122018-02-16
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1021/acssynbio.7b00225
 Degree: -

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Title: ACS Synthetic Biology
  Abbreviation : ACS Synth. Biol.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 7 (2) Sequence Number: - Start / End Page: 443 - 451 Identifier: ISSN: 2161-5063
CoNE: /journals/resource/2161-5063