English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Indirect detection of lactate using CEST in muscle at 7T

Meissner, J.-E., Bauer, C., Korzowski, A., Zaiss, M., Ladd, M., & Bachert, P. (2016). Indirect detection of lactate using CEST in muscle at 7T. Magnetic Resonance Materials in Physics, Biology and Medicine, 29(Supplement 1), S264-S265.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0000-7C26-B Version Permalink: http://hdl.handle.net/21.11116/0000-0000-C852-2
Genre: Meeting Abstract

Files

show Files

Locators

show
hide
Locator:
Link (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Meissner, J-E, Author
Bauer, C, Author
Korzowski, A, Author
Zaiss, M1, 2, Author              
Ladd, ME, Author
Bachert, P, Author
Affiliations:
1Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              
2Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              

Content

show
hide
Free keywords: -
 Abstract: Purpose/Introduction: For cancer and metabolic disorders the concentration of lactate as a metabolite of the anaerobic glycolysis is of particular relevance. Chemical Exchange Saturation Transfer (CEST) MRI enables the non-invasive detection of intracellular lactate1. We demonstrate dynamic measurements of the lactate level after exercise. Employing 31P spectroscopic imaging, it could additionally be shown that the measured effect cannot be solely explained by a change in pH value. Subjects and Methods: It was shown that the hydroxyl proton of lactate yields a CEST effect at 0.4 ppm relative to water1. Employing the metric2 MTRASYM,Lactate = Z(Dx = -0.4 ppm) - Z(Dx =+0.4 ppm) allows to quantify the lactate effect. Imaging: Z–spectra were obtained by centric–reordered 2D–GRE–CEST MRI implemented on a 7–T whole–body scanner (MAGNETOM; Siemens, Germany) using a 28–ch Rx/1–ch Tx 1H knee coil. For saturation 20 Gaussian–shaped rf pulses (tp = 0.1 s, DC = 50 , B1 = 0.9 lT) were applied. 22 adapted frequency offsets were acquired across the water peak resulting in a total acquisition time of 2 min 3 s. After three baseline measurements an exercise was conducted using an MRcompatible foot pedal until exhaustion (*3 min). Subsequent six additional measurements were performed. Results: The total measurement time of close to 2 min allows a dynamic examination of the lactate effect. Figure 1 shows the asymmetry at 0.4 ppm for all 9 measurements. Highlighted in green are the 3 baseline measurements and in red the 6 measurements after exercise. In the mainly used medial gastrocnemius (MG) a decrease of the effect over time can be observed. Discussion/Conclusion: Measuring the MTRASYM at 0.4 ppm allows the non-invasive investigation of lactate in vivo. We were able to measure an increase of lactate effect after plantar dorsi flexion as well as the decrease with time similar to already published work with creatine3. Since pH and lactate effect showed different behaviors in different muscle areas we could exclude pH as a sole cause of the effect. This could allow to study the anaerobic glycolysis in cancer or metabolic disorders.

Details

show
hide
Language(s):
 Dates: 2016-10-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/s10334-016-0570-3
BibTex Citekey: MeissnerBKZLB2016
 Degree: -

Event

show
hide
Title: 33rd Annual Scientific Meeting of the European Society for Magnetic Resonance in Medicine and Biology (ESMRMB 2016)
Place of Event: Wien, Austria
Start-/End Date: -

Legal Case

show

Project information

show

Source 1

show
hide
Title: Magnetic Resonance Materials in Physics, Biology and Medicine
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 29 (Supplement 1) Sequence Number: - Start / End Page: S264 - S265 Identifier: -