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  Expansion microscopy of zebrafish for neuroscience and developmental biology studies

Freifeld, L., Odstrcil, I., Förster, D., Ramirez, A., Gagnon, J. A., Randlett, O., et al. (2017). Expansion microscopy of zebrafish for neuroscience and developmental biology studies. Proceedings of the National Academy of Sciences of the United States of America, 114(50), E10799-E10808. doi:10.1073/pnas.1706281114.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-3D70-D Version Permalink: http://hdl.handle.net/21.11116/0000-0001-3D71-C
Genre: Journal Article

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© 2017 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
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 Creators:
Freifeld, Limor1, Author
Odstrcil, Iris1, Author
Förster, Dominique2, Author              
Ramirez, Alyson1, Author
Gagnon, James A.1, Author
Randlett, Owen1, Author
Costa, Emma K.1, Author
Asano, Shoh1, Author
Celiker, Orhan T.1, Author
Gao, Ruixuan1, Author
Martin-Alarcon, Daniel A.1, Author
Reginato, Paul1, Author
Dick, Cortni1, Author
Chen, Linlin1, Author
Schoppik, David1, Author
Engert, Florian1, Author
Baier, Herwig2, Author              
Boyden, Edward S.1, Author
Affiliations:
1external, ou_persistent22              
2Department: Genes-Circuits-Behavior / Baier, MPI of Neurobiology, Max Planck Society, ou_1128545              

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Free keywords: STRUCTURED ILLUMINATION MICROSCOPY; NUCLEAR-ENVELOPE BREAKDOWN; IN-VIVO; MAUTHNER-CELL; NUCLEOPLASMIC RETICULUM; FLUORESCENT PROTEINS; OPTICAL MICROSCOPY; INTERPHASE NUCLEI; MAMMALIAN-CELLS; NEURAL CIRCUITScience & Technology - Other Topics; zebrafish; superresolution; microscopy; brain;
 Abstract: Expansion microscopy (ExM) allows scalable imaging of preserved 3D biological specimens with nanoscale resolution on fast diffraction-limited microscopes. Here, we explore the utility of ExM in the larval and embryonic zebrafish, an important model organism for the study of neuroscience and development. Regarding neuroscience, we found that ExM enabled the tracing of fine processes of radial glia, which are not resolvable with diffraction-limited microscopy. ExM further resolved putative synaptic connections, as well as molecular differences between densely packed synapses. Finally, ExM could resolve subsynaptic protein organization, such as ring-like structures composed of glycine receptors. Regarding development, we used ExM to characterize the shapes of nuclear invaginations and channels, and to visualize cytoskeletal proteins nearby. We detected nuclear invagination channels at late prophase and telophase, potentially suggesting roles for such channels in cell division. Thus, ExM of the larval and embryonic zebrafish may enable systematic studies of how molecular components are configured in multiple contexts of interest to neuroscience and developmental biology.

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Language(s): eng - English
 Dates: 2017-11-212017-12
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000417806200025
DOI: 10.1073/pnas.1706281114
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proceedings of the National Academy of Sciences of the USA
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 114 (50) Sequence Number: - Start / End Page: E10799 - E10808 Identifier: ISSN: 0027-8424
CoNE: /journals/resource/954925427230