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  In vivo quantification of spatially varying mechanical properties in developing tissues

Serwane, F., Mongera, A., Rowghanian, P., Kealhofer, D., Lucio, A., Hockenbery, Z., et al. (2017). In vivo quantification of spatially varying mechanical properties in developing tissues. Nature methods, 14(2), 181-186. doi:10.1038/nmeth.4101.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-7DE4-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-7DE5-2
Genre: Journal Article

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 Creators:
Serwane, Friedhelm1, 2, Author              
Mongera , Alessandro, Author
Rowghanian , Payan, Author
Kealhofer , David, Author
Lucio , Adam, Author
Hockenbery , Zachary, Author
Campás , Otger, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: The mechanical properties of the cellular microenvironment and their spatiotemporal variations are thought to play a central role in sculpting embryonic tissues, maintaining organ architecture and controlling cell behavior, including cell differentiation. However, no direct in vivo and in situ measurement of mechanical properties within developing 3D tissues and organs has yet been performed. Here we introduce a technique that employs biocompatible, magnetically responsive ferrofluid microdroplets as local mechanical actuators and allows quantitative spatiotemporal measurements of mechanical properties in vivo. Using this technique, we show that vertebrate body elongation entails spatially varying tissue mechanics along the anteroposterior axis. Specifically, we find that the zebrafish tailbud is viscoelastic (elastic below a few seconds and fluid after just 1 min) and displays decreasing stiffness and increasing fluidity toward its posterior elongating region. This method opens new avenues to study mechanobiology in vivo, both in embryogenesis and in disease processes, including cancer.

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Language(s): eng - English
 Dates: 2016-04-272016-10-232016-12-052017-02
 Publication Status: Published in print
 Pages: 9
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 Table of Contents: -
 Rev. Type: Peer
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 14 (2) Sequence Number: - Start / End Page: 181 - 186 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556