English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti

Tavormina, P. L., Kellermann, M. Y., Antony, C. P., Tocheva, E. I., Dalleska, N. F., Jensen, A. J., et al. (2017). Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti. MOLECULAR MICROBIOLOGY, 103(2), 242-252. doi:10.1111/mmi.13553.

Item is

Files

show Files
hide Files
:
Chakkiath_01_17.pdf (Publisher version), 2MB
Name:
Chakkiath_01_17.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Tavormina, Patricia L., Author
Kellermann, Matthias Y., Author
Antony, Chakkiath Paul, Author
Tocheva, Elitza I., Author
Dalleska, Nathan F., Author
Jensen, Ashley J., Author
Valentine, David L., Author
Hinrichs, Kai-Uwe, Author
Jensen, Grant J., Author
Dubilier, Nicole1, Author           
Orphan, Victoria J., Author
Affiliations:
1Department of Symbiosis, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481699              

Content

show
hide
Free keywords: RARE-EARTH-ELEMENTS; INTACT POLAR LIPIDS; OXIDIZING BACTERIA; MASS-SPECTROMETRY; MICROBIAL ECOLOGY; STATIONARY-PHASE; SP NOV.; METHANE; LIFE; RNABiochemistry & Molecular Biology; Microbiology;
 Abstract: In the deep ocean, the conversion of methane into derived carbon and energy drives the establishment of diverse faunal communities. Yet specific biological mechanisms underlying the introduction of methane-derived carbon into the food web remain poorly described, due to a lack of cultured representative deep-sea methanotrophic prokaryotes. Here, the response of the deep-sea aerobic methanotroph Methyloprofundus sedimenti to methane starvation and recovery was characterized. By combining lipid analysis, RNA analysis, and electron cryotomography, it was shown that M. sedimenti undergoes discrete cellular shifts in response to methane starvation, including changes in headgroup-specific fatty acid saturation levels, and reductions in cytoplasmic storage granules. Methane starvation is associated with a significant increase in the abundance of gene transcripts pertinent to methane oxidation. Methane reintroduction to starved cells stimulates a rapid, transient extracellular accumulation of methanol, revealing a way in which methane-derived carbon may be routed to community members. This study provides new understanding of methanotrophic responses to methane starvation and recovery, and lays the initial groundwork to develop Methyloprofundus as a model chemosynthesizing bacterium from the deep sea.

Details

show
hide
Language(s): eng - English
 Dates: 2017
 Publication Status: Issued
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000394691500006
DOI: 10.1111/mmi.13553
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: MOLECULAR MICROBIOLOGY
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 103 (2) Sequence Number: - Start / End Page: 242 - 252 Identifier: ISSN: 0950-382X