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  NURR1 Downregulation Favors Osteoblastic Differentiation of MSCs

Di Benedetto, A., Posa, F., Carbone, C., Cantore, S., Brunetti, G., Centonze, M., et al. (2017). NURR1 Downregulation Favors Osteoblastic Differentiation of MSCs. Stem cells international, 2017: 7617048, pp. 1-11. doi:10.1155/2017/7617048.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-8182-A Version Permalink: http://hdl.handle.net/21.11116/0000-0000-8183-9
Genre: Journal Article

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Di Benedetto, Adriana, Author
Posa , Francesca, Author
Carbone , Claudia, Author
Cantore , Stefania, Author
Brunetti , Giacomina, Author
Centonze , Matteo, Author
Grano , Maria, Author
Muzio , Lorenzo Lo, Author
Cavalcanti-Adam, Elisabetta Ada1, Author              
Mori , Giorgio, Author
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1Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Mesenchymal stem cells (MSCs) have been identified in human dental tissues. Dental pulp stem cells (DPSCs) were classified within MSC family, are multipotent, can be isolated from adult teeth, and have been shown to differentiate, under particular conditions, into various cell types including osteoblasts. In this work, we investigated how the differentiation process of DPSCs toward osteoblasts is controlled. Recent literature data attributed to the nuclear receptor related 1 (NURR1), a still unclarified role in osteoblast differentiation, while NURR1 is primarily involved in dopaminergic neuron differentiation and activity. Thus, in order to verify if NURR1 had a role in DPSC osteoblastic differentiation, we silenced it during all the processes and compared the expression of the main osteoblastic markers with control cultures. Our results showed that the inhibition of NURR1 significantly increased the expression of osteoblast markers collagen I and alkaline phosphatase. Further, in long time cultures, the mineral matrix deposition was strongly enhanced in NURR1-silenced cultures. These results suggest that NURR1 plays a key role in switching DPSC differentiation toward osteoblasts rather than neuronal or even other cell lines. In conclusion, DPSCs represent a source of osteoblast-like cells and downregulation of NURR1 strongly prompted their differentiation toward the osteoblastogenesis process.

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Language(s): eng - English
 Dates: 2017-04-302017-06-122017-07-09
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1155/2017/7617048
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Title: Stem cells international
Source Genre: Journal
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Publ. Info: London [u.a.] : Sage-Hindawi
Pages: - Volume / Issue: 2017 Sequence Number: 7617048 Start / End Page: 1 - 11 Identifier: ISSN: 1687-9678