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  Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach

Pohl, T., Schwab, E., & Cavalcanti-Adam, E. A. (2013). Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach. Journal of visualized experiments.

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 Creators:
Pohl, Theresa1, Author              
Schwab, Elisabeth1, 2, Author              
Cavalcanti-Adam, Elisabetta Ada1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
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Title: Journal of visualized experiments
Source Genre: Journal
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Publ. Info: United States : Boston, Mass. : MYJoVE Corporation, 2006
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1940-087X