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  RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis

Secchi, A., Grigoriou, V., Shapiro, I., Cavalcanti-Adam, E. A., Composto, R., Ducheyne, P., et al. (2007). RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis. Journal of Biomedical Materials Research Part A, 83(3), 577-584. doi:10.1002/jbm.a.31007.

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JBiomedMaterialsResA_83_2007_577.pdf (Any fulltext), 471KB
 
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 Creators:
Secchi , A.G., Author
Grigoriou , V., Author
Shapiro , I.M., Author
Cavalcanti-Adam, Elisabetta Ada1, 2, Author           
Composto , R.J., Author
Ducheyne , P., Author
Adams , C.S., Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: titanium; RGD peptides; apoptosis; osteoblast; APTS
 Abstract: A major cause of implant failure in skeletal tissues is failure of osseointegration, often due to lack of adhesion of cells to the titanium (Ti) alloy interface. Since arginine-glycine-aspartic acid (RGD)-containing peptides have been shown to regulate osteoblast adhesion, we tested the hypothesis that, bound to a Ti surface, these peptides would promote osteoblasts differentiation, while at the same time inhibit apoptosis. RGDS and RGES (control) peptides were covalently linked to Ti discs using an APTS linker. While the grafting of both RGDS and RGES significantly increased Ti surface roughness, contact angle analysis showed that APTS significantly increased the surface hydrophobicity; when the peptides were tethered to Ti, this was reduced. To evaluate attachment, MC3T3-E1 osteoblast cells were grown on these discs. Significantly more cells attached to the Ti-grafted RGDS then the Ti-grafted RGES control. Furthermore, expression of the osteoblasts phenotype was significantly enhanced on the Ti-grafted RGDS surface. When cells attached to the Ti-grafted RGDS were challenged with staurosporine, an apoptogen, there was significant inhibition of apoptosis; in contrast, osteoblasts adherent to the Ti-grafted RGES were killed. It is concluded that RGD-containing peptides covalently bonded to Ti promotes osteoblasts attachment and survival with minimal changes to the surface of the alloy. Therefore, such modifications to Ti would have the potential to promote osseointegration in vivo.

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Language(s): eng - English
 Dates: 2006-05-192006-02-012006-06-162006-05-192007-12-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Journal of Biomedical Materials Research Part A
Source Genre: Journal
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Publ. Info: Hoboken, NJ : John Wiley & Sons
Pages: - Volume / Issue: 83 (3) Sequence Number: - Start / End Page: 577 - 584 Identifier: ISSN: 1549-3296
CoNE: https://pure.mpg.de/cone/journals/resource/954925411829_1