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  A single NaK channel conformation is not enough for non-selective ion conduction.

Shi, C., He, Y., Hendriks, K., de Groot, B. L., Cai, X., Tian, C., et al. (2018). A single NaK channel conformation is not enough for non-selective ion conduction. Nature Communications, 9: 717. doi:10.1038/s41467-018-03179-y.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-84B0-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-B95C-6
Genre: Journal Article

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 Creators:
Shi, C., Author
He, Y., Author
Hendriks, K., Author
de Groot, B. L.1, Author              
Cai, X., Author
Tian, C., Author
Lange, A., Author
Sun, H., Author
Affiliations:
1Research Group of Computational Biomolecular Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578573              

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 Abstract: NaK and other non-selective channels are able to conduct both sodium (Na+) and potassium (K+) with equally high efficiency. In contrast to previous crystallographic results, we show that the selectivity filter (SF) of NaK in native-like lipid membranes adopts two distinct conformations that are stabilized by either Na+ or K+ ions. The atomic differences of these conformations are resolved by solid-state NMR (ssNMR) spectroscopy and molecular dynamics (MD) simulations. Besides the canonical K+ permeation pathway, we identify a side entry ion-conduction pathway for Na+ permeation unique to NaK. Moreover, under otherwise identical conditions ssNMR spectra of the K+ selective NaK mutant (NaK2K) reveal only a single conformational state. Therefore, we propose that structural plasticity within the SF and the selection of these conformations by different ions are key molecular determinants for highly efficient conduction of different ions in non-selective cation channels.

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Language(s): eng - English
 Dates: 2018-02-19
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-018-03179-y
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Title: Nature Communications
Source Genre: Journal
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Pages: 8 Volume / Issue: 9 Sequence Number: 717 Start / End Page: - Identifier: -