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  Expression of vimentin and nuclear lamins during the in vitro differentiation of human promyelocytic leukemia cells HL-60

Paulin−Levasseur, M., Giese, G., Scherbarth, A., & Traub, P. (1989). Expression of vimentin and nuclear lamins during the in vitro differentiation of human promyelocytic leukemia cells HL-60. European Journal of Cell Biology: EJCB, 50(2), 453-461. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2697560.

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EurJCellBiol_50_1989_453.pdf (Any fulltext), 4MB
 
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Paulin−Levasseur, Micheline, Author
Giese, Günter1, 2, Author              
Scherbarth, Annemarie1, 2, Author              
Traub, Peter, Author
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Light Microscopy Facility, Max Planck Institute for Medical Research, Max Planck Society, ou_1497720              

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Free keywords: Vimentin; nuclear lamins; differentiation; phobol ester; promyelocytic leukemia cells; HL-60 cells
 Abstract: We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expression of vimentin and lamins A and C during differentiation of these tumor cells along the macrophage or granulocytic pathway in response to the inducing effects of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) or dimethyl sulfoxide. Our results show that, while the expression of lamin B remains largely unchanged, the synthesis of vimentin and lamins A and C is dramatically enhanced during the maturation of HL-60 cells along both hemopoietic pathways. Northern blot analysis of cellular RNAs isolated from untreated and TPA-treated HL-60 cell populations as well as from control HeLa cells was performed using two oligonucleotides, one complementary to the 5' region common to human lamin A/C mRNAs and the other to the 5' region of hamster vimentin mRNA. Very low but still detectable amounts of vimentin and lamin A/C mRNAs were found in untreated HL-60 cell population, in accordance with the detection of small quantities of vimentin and lamins A and C in these populations. This is probably due to the presence of a small number of spontaneously differentiating cells. On the other hand, strong signals comparable to those obtained with RNA from control HeLa cells were detected for the three mRNA species from TPA-treated cells.(ABSTRACT TRUNCATED AT 250 WORDS).

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Language(s): eng - English
 Dates: 1989-04-251989-09-271989-12
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: European Journal of Cell Biology : EJCB
  Other : Eur. J. Cell Biol.
Source Genre: Journal
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Publ. Info: Stuttgart : Wissenschaftliche Verlagsgesellschaft.
Pages: - Volume / Issue: 50 (2) Sequence Number: - Start / End Page: 453 - 461 Identifier: ISSN: 0070-2463
CoNE: https://pure.mpg.de/cone/journals/resource/954925486755