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  Calcium Sensor for Photoacoustic Imaging

Roberts, S., Seeger, M., Jiang, Y., Mishra, A., Sigmund, F., Stelzl, A., et al. (2018). Calcium Sensor for Photoacoustic Imaging. Journal of the American Chemical Society, 140(8), 2718-2721. doi:10.1021/jacs.7b03064.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-C2B0-D Version Permalink: http://hdl.handle.net/21.11116/0000-0000-F966-5
Genre: Journal Article

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Roberts, S, Author
Seeger, M, Author
Jiang, Y1, Author              
Mishra, A1, Author              
Sigmund, F, Author
Stelzl, A, Author
Lauri, A, Author
Symvoulidis, P, Author
Rolbieski, H, Author
Preller, M, Author
Deán-Ben, XL, Author
Razansky, D, Author
Orschmann, T, Author
Desbordes, SD, Author
Vetschera, P, Author
Bach, T, Author
Ntziachristos, V, Author
Westmeyer, GG, Author
Affiliations:
1Institute for Biological and Medical Imaging and Institute of Developmental Genetics, Helmholtz Zentrum München, ou_persistent22              

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 Abstract: We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.

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 Dates: 2017-092018-02
 Publication Status: Published in print
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 Identifiers: DOI: 10.1021/jacs.7b03064
BibTex Citekey: RobertsSJMSSLSRPDRODVBNW2017
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 140 (8) Sequence Number: - Start / End Page: 2718 - 2721 Identifier: -