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  GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs

Ymer, S., Schofield, P. R., Draguhn, A., Werner, P., Köhler, M., & Seeburg, P. H. (1989). GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs. EMBO Journal, 8(6), 1665-1670. doi:10.1002/j.1460-2075.1989.tb03557.x.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-8E3B-F Version Permalink: http://hdl.handle.net/21.11116/0000-0000-8E3C-E
Genre: Journal Article

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 Creators:
Ymer, Sanie, Author
Schofield, Peter R., Author
Draguhn, Andreas1, Author              
Werner, Pia2, Author              
Köhler, Martin2, Author              
Seeburg, Peter H.2, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: GABAA receptor; β subunit; receptor subtypes; molecular cloning; oocyte expression
 Abstract: Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second transmembrane domain of GABAA receptor subunits. The beta 2 and beta 3 subunits share approximately 72% sequence identity with the previously characterized beta 1 polypeptide. Northern analysis showed that both beta 2 and beta 3 mRNAs are more abundant in the brain than beta 1 mRNA. All three beta subunit encoding cDNAs were also identified in a library constructed from adrenal medulla RNA. Each beta subunit, when co-expressed in Xenopus oocytes with an alpha subunit, forms functional GABAA receptors. These results, together with the known alpha subunit heterogeneity, suggest that a variety of related but functionally distinct GABAA receptor subtypes are generated by different subunit combinations.

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Language(s): eng - English
 Dates: 1989-03-031989-06-011989-06-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 8 (6) Sequence Number: - Start / End Page: 1665 - 1670 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061