English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development

Nahorski, M. S., Borner, G. H. H., Shaikh, S. S., Davies, A. K., Al-Gazali, L., Antrobus, R., et al. (2018). Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development. Scientific Reports, 8: 2340. doi:10.1038/s41598-018-19980-0.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
s41598-018-19980-0.pdf (Publisher version), 4MB
Name:
s41598-018-19980-0.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
Open Access
:
41598_2018_19980_MOESM1_ESM.doc (Supplementary material), 5MB
Name:
41598_2018_19980_MOESM1_ESM.doc
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/msword / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
41598_2018_19980_MOESM2_ESM.xlsx (Supplementary material), 30KB
Name:
41598_2018_19980_MOESM2_ESM.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Nahorski, Michael S.1, Author
Borner, Georg H. H.2, Author              
Shaikh, Samiha S.1, Author
Davies, Alexandra K.1, Author
Al-Gazali, Lihadh1, Author
Antrobus, Robin1, Author
Woods, C. Geoffrey1, Author
Affiliations:
1external, ou_persistent22              
2Borner, Georg / Systems Biology of Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_3060205              

Content

show
hide
Free keywords: NEUROTROPHIC FACTOR; CELL-DEATH; PROTEIN; CHC22; PAIN; MECHANISMS; GENE; BAFILOMYCIN; AUTOPHAGY; DISTINCTScience & Technology - Other Topics;
 Abstract: The repertoire of cell types in the human nervous system arises through a highly orchestrated process, the complexity of which is still being discovered. Here, we present evidence that CHC22 has a non-redundant role in an early stage of neural precursor differentiation, providing a potential explanation of why CHC22 deficient patients are unable to feel touch or pain. We show the CHC22 effect on neural differentiation is independent of the more common clathrin heavy chain CHC17, and that CHC22-dependent differentiation is mediated through an autocrine/paracrine mechanism. Using quantitative proteomics, we define the composition of clathrin-coated vesicles in SH-SY5Y cells, and determine proteome changes induced by CHC22 depletion. In the absence of CHC22 a subset of dense core granule (DCG) neuropeptides accumulated, were processed into biologically active 'mature' forms, and secreted in sufficient quantity to trigger neural differentiation. When CHC22 is present, however, these DCG neuropeptides are directed to the lysosome and degraded, thus preventing differentiation. This suggests that the brief reduction seen in CHC22 expression in sensory neural precursors may license a step in neuron precursor neurodevelopment; and that this step is mediated through control of a novel neuropeptide processing pathway.

Details

show
hide
Language(s): eng - English
 Dates: 2018-02-05
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 2340 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322