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Free keywords:
NF-KAPPA-B; QUALITY-CONTROL; NEURODEGENERATIVE DISEASES; IDENTIFIES
YOS9P; DEGRADATION; OS-9; ER; EXPRESSION; CHAPERONE; PATHWAYBiochemistry & Molecular Biology; Cell Biology; endoplasmic reticulum; protein aggregation; proteostasis; quality
control;
Abstract:
Protein aggregation is associated with neurodegeneration and various other pathologies. How specific cellular environments modulate the aggregation of disease proteins is not well understood. Here, we investigated how the endoplasmic reticulum (ER) quality control system handles beta-sheet proteins that were designed de novo to form amyloid-like fibrils. While these proteins undergo toxic aggregation in the cytosol, we find that targeting them to the ER (ER-beta) strongly reduces their toxicity. ER-beta is retained within the ER in a soluble, polymeric state, despite reaching very high concentrations exceeding those of ER-resident molecular chaperones. ER-beta is not removed by ER-associated degradation (ERAD) but interferes with ERAD of other proteins. These findings demonstrate a remarkable capacity of the ER to prevent the formation of insoluble beta-aggregates and the secretion of potentially toxic protein species. Our results also suggest a generic mechanism by which proteins with exposed b-sheet structure in the ER interfere with proteostasis.
