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  In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

Lemke, S. B., & Schnorrer, F. (2018). In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae. Journal of Visualized Experiments, (132): e57312. doi:10.3791/57312.

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© 2018 Creative Commons Attribution-NonCommercial-NoDerivs 3.0
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 Creators:
Lemke, Sandra B.1, Author              
Schnorrer, Frank1, Author              
Affiliations:
1Schnorrer, Frank / Muscle Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565168              

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 Abstract: Muscles together with tendons and the skeleton enable animals including humans to move their body parts. Muscle morphogenesis is highly conserved from animals to humans. Therefore, the powerful Drosophila model system can be used to study concepts of muscle-tendon development that can also be applied to human muscle biology. Here, we describe in detail how morphogenesis of the adult muscle-tendon system can be easily imaged in living, developing Drosophila pupae. Hence, the method allows investigating proteins, cells and tissues in their physiological environment. In addition to a step-by-step protocol with helpful tips, we provide a comprehensive overview of fluorescently tagged marker proteins that are suitable for studying the muscle-tendon system. To highlight the versatile applications of the protocol, we show example movies ranging from visualization of long-term morphogenetic events – occurring on the time scale of hours and days – to visualization of short-term dynamic processes like muscle twitching occurring on time scale of seconds. Taken together, this protocol should enable the reader to design and perform live-imaging experiments for investigating muscle-tendon morphogenesis in the intact organism.

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 Dates: 2018
 Publication Status: Published online
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 Identifiers: DOI: 10.3791/57312
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Title: Journal of Visualized Experiments
  Other : Journal of Visualized Experiments: JoVE
  Abbreviation : J. Vis. Exp.
Source Genre: Journal
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Publ. Info: Rockville Pike, Bethesda MD : JoVE
Pages: - Volume / Issue: (132) Sequence Number: e57312 Start / End Page: - Identifier: ISSN: 1940-087X
CoNE: https://pure.mpg.de/cone/journals/resource/1940087X