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  Spherical network contraction forms microtubule asters in confinement

Juniper, M., Weiss, M., Platzman, I., Spatz, J. P., & Surrey, T. (2018). Spherical network contraction forms microtubule asters in confinement. Soft Matter, 14(6), 901-909. doi:10.1039/C7SM01718A.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-B907-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-8AB6-6
Genre: Journal Article

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 Creators:
Juniper, Michael, Author
Weiss, Marian, Author
Platzman, Ilia1, 2, Author              
Spatz, Joachim P.1, 2, Author              
Surrey, Thomas, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Microtubules and motor proteins form active filament networks that are critical for a variety of functions in living cells. Network topology and dynamics are the result of a self-organisation process that takes place within the boundaries of the cell. Previous biochemical in vitro studies with biomimetic systems consisting of purified motors and microtubules have demonstrated that confinement has an important effect on the outcome of the self-organisation process. However, the pathway of motor/microtubule self-organisation under confinement and its effects on network morphology are still poorly understood. Here, we have investigated how minus-end directed microtubule cross-linking kinesins organise microtubules inside polymer-stabilised microfluidic droplets of well-controlled size. We find that confinement can impose a novel pathway of microtubule aster formation proceeding via the constriction of an initially spherical motor/microtubule network. This mechanism illustrates the close relationship between confinement, network contraction, and aster formation. The spherical constriction pathway robustly produces single, well-centred asters with remarkable reproducibility across thousands of droplets. These results show that the additional constraint of well-defined confinement can improve the robustness of active network self-organisation, providing insight into the design principles of self-organising active networks in micro-scale confinement.

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Language(s): eng - English
 Dates: 2017-08-252017-10-262018-01-24
 Publication Status: Published in print
 Pages: 9
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 Rev. Method: Peer
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Title: Soft Matter
  Abbreviation : Soft Matter
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 14 (6) Sequence Number: - Start / End Page: 901 - 909 Identifier: ISSN: 1744-683X
CoNE: https://pure.mpg.de/cone/journals/resource/1744-683X