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  Transient expression shows ligand gating and allosteric potentiation of GABAA receptor subunits

Pritchett, D. B., Sontheimer, H., Gorman, C. M., Kettenmann, H., Seeburg, P. H., & Schofield, P. R. (1988). Transient expression shows ligand gating and allosteric potentiation of GABAA receptor subunits. Science, 242(4883), 1306-1309. doi:10.1126/science.2848320.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-B9AF-B Version Permalink: https://hdl.handle.net/21.11116/0000-0000-B9B0-8
Genre: Journal Article
Alternative Title : Ligand−gating and allosteric potentiation of human GABA receptor subunits in a transient mammalian cell expression system

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 Creators:
Pritchett, Dolan B., Author
Sontheimer, Harald, Author
Gorman, C. M., Author
Kettenmann, Helmut, Author
Seeburg, Peter H.1, Author           
Schofield, Peter R., Author
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1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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 Abstract: Human gamma-aminobutyric acid A (GABAA) receptor subunits were expressed transiently in cultured mammalian cells. This expression system allows the simultaneous characterization of ligand-gated ion channels by electrophysiology and by pharmacology. Thus, coexpression of the alpha and beta subunits of the GABAA receptor generated GABA-gated chloride channels and binding sites for GABAA receptor ligands. Channels consisting of only alpha or beta subunits could also be detected. These homomeric channels formed with reduced efficiencies compared to the heteromeric receptors. Both of these homomeric GABA-responsive channels were potentiated by barbiturate, indicating that sites for both ligand-gating and allosteric potentiation are present on receptors assembled from either subunit.

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Language(s): eng - English
 Dates: 1988-12-02
 Publication Status: Issued
 Pages: 3
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.2848320
URI: https://www.ncbi.nlm.nih.gov/pubmed/2848320
URI: https://www.ncbi.nlm.nih.gov/m/pubmed/2848320/
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Title: Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 242 (4883) Sequence Number: - Start / End Page: 1306 - 1309 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1