English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The human growth hormone gene locus: structure, evolution, and allelic variations

Hirt, H., Kimelman, J., Birnbaum, M. J., Chen, E. Y., Seeburg, P. H., Eberhardt, N. L., et al. (1987). The human growth hormone gene locus: structure, evolution, and allelic variations. DNA, 6(1), 59-70. doi:10.1089/dna.1987.6.59.

Item is

Files

show Files
hide Files
:
DNA_6_1987_59.pdf (Any fulltext), 6MB
 
File Permalink:
-
Name:
DNA_6_1987_59.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://doi.org/10.1089/dna.1987.6.59 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Hirt, Heribert, Author
Kimelman, Judy, Author
Birnbaum, Morris J., Author
Chen, Ellson Y., Author
Seeburg, Peter H.1, Author           
Eberhardt, Norman L., Author
Barta, Andrea, Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

Content

show
hide
Free keywords: -
 Abstract: Genomic clones containing the closely related genes for human growth hormone (hGH) and chorionic somatomammotropin (hCS) were obtained from genomic bacteriophage lambda and cosmid libraries. The entire GH/CS chromosomal locus was reconstructed utilizing overlapping restriction fragments characterized from the isolated clones. The hGH/hCS locus contains two GH genes and three CS genes spanning 48 kb of DNA in the order: 5'-(hGH-1/hCS-5/hCS-1/hGH-2/hCS-2)-3', confirming analysis of cosmid clones obtained from a different human library (Barsh et al., 1983). To complete the characterization of the hCS genes, the nucleotide sequence of the hCS-5 gene was determined. Sequence analysis revealed a mutation of the 5' splice site at the exon II-intron B boundary, suggesting that the hCS-5 gene is a pseudogene. The nucleotide sequence of an allelic variant of the hCS-2 gene was determined and found to contain a single amino acid substitution and the deletion of a single codon. The hGH/hCS gene locus was further characterized by the localization of at least 27 Alu-type repetitive sequences and identification of three unique sequences in the vicinity of several hGH and hCS genes which define the probable breakpoints of the evolutionary duplication units. These data, combined with the nucleotide sequences of all five GH and CS genes, indicate that the hGH/hCS gene locus has evolved by duplication mechanisms. Evidence for the occurrence of at least one gene conversion event involving the hCS-1 gene precursor and the hCS-2 gene was found, indicating that the hGH/hCS gene locus has evolved by concerted mechanisms. The structure of the hCS genes is discussed in light of recent studies of CS genes from other mammalian species.

Details

show
hide
Language(s): eng - English
 Dates: 2009-03-251987-02
 Publication Status: Issued
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: DNA
  Other : DNA
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York : Mary Ann Liebert
Pages: - Volume / Issue: 6 (1) Sequence Number: - Start / End Page: 59 - 70 Identifier: ISSN: 0198-0238
CoNE: https://pure.mpg.de/cone/journals/resource/954925275481