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  Identification and characterization of G90, a novel mouse RNA that lacks an extensive open reading frame

Krause, R., Hemberger, M., Himmelbauer, H., Kalscheuer, V., & Fundele, R. H. (1999). Identification and characterization of G90, a novel mouse RNA that lacks an extensive open reading frame. Gene, 232(1), 35-42.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0002-E027-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-E028-4
Genre: Journal Article

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 Creators:
Krause, R.1, Author
Hemberger, M.1, Author
Himmelbauer, H.1, Author
Kalscheuer, V.1, Author
Fundele, R. H.1, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: Amino Acid Sequence Animals Base Sequence Blotting, Northern Chromosome Mapping DNA, Complementary/genetics *Gene Expression In Situ Hybridization, Fluorescence Intestine, Large/*metabolism Intestine, Small/*metabolism Mice Molecular Sequence Data Open Reading Frames/*genetics Poly A/metabolism RNA/*genetics/metabolism Sequence Analysis, DNA
 Abstract: We describe the cloning and characterization of the murine G90 gene, identified by subtractive hybridization based on the differential presence of its transcript in large and small intestine. The full-length cDNA and genomic sequences were cloned and found to produce a 1.5kb transcript that is polyadenylated but has no open reading frame larger than 249bp. The G90 gene was mapped to the proximal region of mouse chromosome 6. Expression analysis by Northern blotting showed that G90 is transcribed at very high levels in the small intestine and at lower levels in large intestine, testis and kidney of the mouse. In situ hybridization analysis on sections of small and large intestine and testis showed that G90 transcripts are present only in post-mitotic cells.

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 Dates: 1999
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: Other: 10333519
ISSN: 0378-1119 (Print) 0378-1119 (Linking)
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Title: Gene
  Alternative Title : Gene
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 232 (1) Sequence Number: - Start / End Page: 35 - 42 Identifier: -