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  Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh in Bacillus subtilis.

Dickmanns, A., Zschiedrich, C. P., Arens, J., Parfentev, I., Gundlach, J., Hofele, R., et al. (2018). Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh in Bacillus subtilis. The Journal of Biological Chemistry, 293(16), 5781-5792. doi:10.1074/jbc.RA117.001289.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-CD29-C Version Permalink: http://hdl.handle.net/21.11116/0000-0003-48CD-6
Genre: Journal Article

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 Creators:
Dickmanns, A., Author
Zschiedrich, C. P., Author
Arens, J., Author
Parfentev, I.1, Author              
Gundlach, J., Author
Hofele, R.1, Author              
Neumann, P., Author
Urlaub, H.1, Author              
Görke, B., Author
Ficner, R., Author
Stulke, J., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: Bacillus; Crh; bacteria; bacterial genetics; bacterial metabolism; catabolite regulation; crystal structure; glycolysis; metabolic regulation; methylglyoxal synthase; phosphoprotein; prokaryotic signal-transduction; protein cross-linking; protein structure; protein-protein interaction
 Abstract: Utilization of energy-rich carbon sources such as glucose is fundamental to the evolutionary success of bacteria. Glucose can be catabolized via glycolysis for feeding the intermediary metabolism. The methylglyoxal synthase MgsA produces methylglyoxal from the glycolytic intermediate dihydroxyacetone phosphate. Methylglyoxal is toxic, requiring stringent regulation of MgsA activity. In the Gram-positive bacterium Bacillus subtilis, an interaction with the phosphoprotein Crh controls MgsA activity. In the absence of preferred carbon sources, Crh is present in the nonphosphorylated state and binds to and thereby inhibits MgsA. To better understand the mechanism of regulation of MgsA, here we performed biochemical and structural analyses of B. subtilis MgsA and of its interaction with Crh. Our results indicated that MgsA forms a hexamer (i.e. a trimer of dimers) in the crystal structure, whereas it seems to exist in an equilibrium between a dimer and hexamer in solution. In the hexamer, two alternative dimers could be distinguished, but only one appeared to prevail in solution. Further analysis strongly suggested that the hexamer is the biologically active form. In vitro cross-linking studies revealed that Crh interacts with the N-terminal helices of MgsA and that the Crh-MgsA binding inactivates MgsA by distorting and thereby blocking its active site. In summary, our results indicate that dimeric and hexameric MgsA species exist in an equilibrium in solution, that the hexameric species is the active form, and that binding to Crh deforms and blocks the active site in MgsA.

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Language(s): eng - English
 Dates: 2018-03-072018-04-20
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1074/jbc.RA117.001289
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Title: The Journal of Biological Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 293 (16) Sequence Number: - Start / End Page: 5781 - 5792 Identifier: -