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  Regulation of methionine synthesis in Escherichia coli: effect of metJ gene product and S-adenosylmethionine on the in vitro expression of the metB, metL and metJ genes

Shoeman, R. L., Coleman, T., Redfield, B., Greene, R. C., Smith, A. A., Saint−Girons, I., et al. (1985). Regulation of methionine synthesis in Escherichia coli: effect of metJ gene product and S-adenosylmethionine on the in vitro expression of the metB, metL and metJ genes. Biochemical and Biophysical Research Communications, 133(2), 731-739. doi:10.1016/0006-291X(85)90965-9.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-CEC0-F Version Permalink: https://hdl.handle.net/21.11116/0000-0000-CEC1-E
Genre: Journal Article

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BiochemBiophysResComm_133_1986_731.pdf (Any fulltext), 722KB
 
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 Creators:
Shoeman, Robert L.1, 2, 3, Author           
Coleman, Timothy, Author
Redfield, Betty, Author
Greene, Ronald C., Author
Smith, Albert A., Author
Saint−Girons, Isabelle, Author
Brot, Nathan, Author
Weissbach, Herbert, Author
Affiliations:
1Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society, ou_1497692              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
3Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497690              

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 Abstract: The regulation of the expression of three Escherichia coli met genes, metB, which codes for cystathionine gamma-synthetase (EC 4.2.99.9), metL, which codes for aspartokinase II-homoserine dehydrogenase II (EC 2.7.2.4-EC 1.1.1.3) and metJ, which codes for the methionine regulon aporepressor, has been studied using highly purified DNA-directed in vitro protein synthesis systems. In a system where the entire gene product is synthesized, the expression of the metB and metL genes is specifically inhibited by MetJ protein (repressor protein) and S-adenosylmethionine (AdoMet). In a simplified system that measures the formation of the first dipeptide of the gene product (fMet-Ala for the metJ gene), MetJ protein and AdoMet partially repress (approximately 40-60%) metJ gene expression. Thus, the metJ gene can be partially autoregulated by its gene product.

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Language(s): eng - English
 Dates: 1985-10-282004-12-021985-12-17
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0006-291X(85)90965-9
URI: https://www.ncbi.nlm.nih.gov/pubmed/3910040
 Degree: -

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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 133 (2) Sequence Number: - Start / End Page: 731 - 739 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1