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  Combined multi-plane phase retrieval and super-resolution optical fluctuation imaging for 4D cell microscopy.

Descloux, A., Grussmayer, K. S., Bostan, E., Lukes, T., Bouwens, A., Sharipov, A., et al. (2018). Combined multi-plane phase retrieval and super-resolution optical fluctuation imaging for 4D cell microscopy. Nature Photonics, 12(3), 165-172. doi:10.1038/s41566-018-0109-4.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-D01D-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-47A0-8
Genre: Journal Article

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 Creators:
Descloux, A., Author
Grussmayer, K. S., Author
Bostan, E., Author
Lukes, T., Author
Bouwens, A, Author
Sharipov, A., Author
Geissbuehler, S., Author
Mahul-Mellier, A. L., Author
Lashuel, H. A., Author
Leutenegger, M.1, Author              
Lasser, T., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going 'beyond the diffraction barrier' comes at a price, since most far-field super-resolution imaging techniques trade temporal for spatial super-resolution. We propose the combination of a novel label-free white light quantitative phase imaging with fluorescence to provide high-speed imaging and spatial super-resolution. The non-iterative phase retrieval relies on the acquisition of single images at each z-location and thus enables straightforward 3D phase imaging using a classical microscope. We realized multi-plane imaging using a customized prism for the simultaneous acquisition of eight planes. This allowed us to not only image live cells in 3D at up to 200 Hz, but also to integrate fluorescence super-resolution optical fluctuation imaging within the same optical instrument. The 4D microscope platform unifies the sensitivity and high temporal resolution of phase imaging with the specificity and high spatial resolution of fluorescence microscopy.

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Language(s): eng - English
 Dates: 2018-02-262018-03
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41566-018-0109-4
 Degree: -

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Title: Nature Photonics
Source Genre: Journal
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Pages: - Volume / Issue: 12 (3) Sequence Number: - Start / End Page: 165 - 172 Identifier: -