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  Protein fusions with the kanamycin resistance gene from transposon Tn5

Reiss, B., Sprengel, R., & Schaller, H. (1984). Protein fusions with the kanamycin resistance gene from transposon Tn5. EMBO Journal, 3(13), 3317-3322. doi:10.1002/j.1460-2075.1984.tb02297.x.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-D39E-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-D39F-F
Genre: Journal Article

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EMBOJ_3_1984_3317.pdf (Any fulltext), 2MB
 
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 Creators:
Reiss, Bernd, Author
Sprengel, Rolf1, 2, 3, Author              
Schaller, Heinz, Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497741              
3Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society, ou_1497733              

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Free keywords: expression in E. coli; fusion proteins; neomycin phosphotransferase II; protein processing
 Abstract: The gene for the neomycin phosphotransferase II (NPT II) from transposon Tn5 was fused at the amino or carboxy terminus to foreign DNA sequences coding for 3-300 amino acids and the properties of the fused proteins were investigated. All amino-terminal fusions examined conferred kanamycin resistance to their host cell, but profound differences in their enzymatic activity and stability were detected. Short additions to the amino terminus of the NPT II resulted in highly enzymatically active fusion proteins whereas long amino-terminal fusions often had to be proteolytically degraded to release active proteins. Fusions at the carboxy-terminal end of the NPT II protein did not always induce kanamycin resistance and their enzymatic activity depended more stringently on the nature of the junction sequence.

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Language(s): eng - English
 Dates: 1984-10-161984-12-20
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 3 (13) Sequence Number: - Start / End Page: 3317 - 3322 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061