English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI.

Weill, U., Arakel, E. C., Goldmann, O., Golan, M., Chuartzman, S., Munro, S., et al. (2018). Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI. Traffic, 19(5), 370-379. doi:10.1111/tra.12560.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0000-D4C9-E Version Permalink: http://hdl.handle.net/21.11116/0000-0003-C220-D
Genre: Journal Article

Files

show Files
hide Files
:
2561045.pdf (Publisher version), 13MB
 
File Permalink:
-
Name:
2561045.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), MBPC; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2561045_Suppl_1.pdf (Supplementary material), 202KB
Name:
2561045_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2561045_Suppl_2.pdf (Supplementary material), 651KB
Name:
2561045_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2561045_Suppl_3.xlsx (Supplementary material), 66KB
Name:
2561045_Suppl_3.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2561045_Suppl_4.xlsx (Supplementary material), 44KB
Name:
2561045_Suppl_4.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Weill, U., Author
Arakel, E. C., Author
Goldmann, O., Author
Golan, M., Author
Chuartzman, S., Author
Munro, S., Author
Schwappach, B.1, Author              
Schuldiner, M., Author
Affiliations:
1Max Planck Fellow Blanche Schwappach, ou_1548137              

Content

show
hide
Free keywords: cargo, COPI, endomembrane, Lam5, SWAT library, yeast
 Abstract: A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with seven known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the Endoplasmic Reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components.

Details

show
hide
Language(s): eng - English
 Dates: 2018-03-122018-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Internal
 Identifiers: DOI: 10.1111/tra.12560
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Traffic
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 19 (5) Sequence Number: - Start / End Page: 370 - 379 Identifier: -