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  Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions

Malik-Sheriff, R. S., Imtiaz, S., Grecco, H. E., & Zamir, E. (2018). Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions. Scientific Reports, 8: 2187, pp. 1-15. doi:10.1038/s41598-018-20252-0.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-D864-C Version Permalink: http://hdl.handle.net/21.11116/0000-0001-DB8D-A
Genre: Journal Article

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 Creators:
Malik-Sheriff, Rahuman S., Author              
Imtiaz, Sarah, Author              
Grecco, Hernán E., Author              
Zamir, Eli1, Author              
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1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: Focal adhesions anchor contractile actin fibers with the extracellular matrix, sense the generated tension and respond to it by changing their morphology and composition. Here we ask how this mechanosensing is enabled at the protein-network level, given the modular assembly and multitasking of focal adhesions. To address this, we applied a sensitive 4-color live cell imaging approach, enabling monitoring patterns of molecular changes in single focal adhesions. Co-imaging zyxin, FAK, vinculin and paxillin revealed heterogeneities in their responses to Rho-associated kinase (ROCK)-mediated perturbations of actomyosin contractility. These responses were rather weakly correlated between the proteins, reflecting diverse compositional changes in different focal adhesions. This diversity is partially attributable to the location of focal adhesions, their area, molecular content and previous contractility perturbations, suggesting that integration of multiple local cues shapes differentially focal adhesion mechano-responsiveness. Importantly, the compositional changes upon ROCK perturbations exhibited distinct paths in different focal adhesions. Moreover, the protein exhibiting the strongest response to ROCK perturbations varied among different focal adhesions. The diversity in response patterns is plausibly enabled by the modular mode of focal adhesions assembly and can provide them the needed flexibility to perform multiple tasks by combining optimally a common set of multifunctional components.

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Language(s): eng - English
 Dates: 2016-11-212017-12-272018-02-01
 Publication Status: Published online
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41598-018-20252-0
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 2187 Start / End Page: 1 - 15 Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322