English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli

Pennica, D., Holmes, K. C., Kohr, W. J., Harkins, R. N., Vehar, G. A., Ward, C. A., et al. (1983). Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature, 301(5897), 214-221. doi:10.1038/301214a0.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-0000-DB45-C Version Permalink: https://hdl.handle.net/21.11116/0000-0000-DB46-B
Genre: Journal Article

Files

show Files
hide Files
:
Nature_301_1983_214.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
Nature_301_1983_214.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://www.nature.com/articles/301214a0.pdf (Any fulltext)
Description:
-
OA-Status:
Locator:
https://doi.org/10.1038/301214a0 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Pennica, Diane, Author
Holmes, Kenneth C.1, 2, 3, Author           
Kohr, William J., Author
Harkins, Richard N., Author
Vehar, Gordon A., Author
Ward, Carole A., Author
Bennett, William F., Author
Ylverton, E., Author
Seeburg, Peter H.4, Author           
Heyneker , Herbert L., Author
Goeddel, David V., Author
Collen, Desire, Author
Affiliations:
1Protein Cristallography XDS, Max Planck Institute for Medical Research, Max Planck Society, ou_1497735              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
3Muscle Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497731              
4Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

Content

show
hide
Free keywords: -
 Abstract: Bacterial clones containing human tissue-type plasminogen activator (t-PA) cDNA sequences were identified in a cDNA library prepared using gel-fractionated mRNA from human melanoma cells. A plasmid containing the Escherichia coli trp promoter and the cDNA sequence coding f or the 527-amino acid mature t-PA protein was constructed for expression in E. coli. A polypeptide was produced having the fibrinolytic properties characteristic of authentic human t-PA.

Details

show
hide
Language(s): eng - English
 Dates: 1982-10-121982-11-231983-01-201983-01-20
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/301214a0
URI: https://www.ncbi.nlm.nih.gov/pubmed/6337343
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature
  Abbreviation : Nature
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 301 (5897) Sequence Number: - Start / End Page: 214 - 221 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238