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  Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions

Sprengel, A., Lill, P., Stegemann, P., Bravo-Rodriguez, K., Schöneweiß, E.-C., Merdanovic, M., et al. (2017). Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions. Nature Communications, 8: 14472 (2017). doi:10.1038/ncomms14472.

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Sprengel, Andreas1, Autor
Lill, Pascal2, Autor
Stegemann, Pierre1, Autor
Bravo-Rodriguez, Kenny3, Autor           
Schöneweiß, Elisa-C.1, Autor
Merdanovic, Melisa1, Autor
Gudnason, Daniel4, Autor
Aznauryan, Mikayel4, Autor
Gamrad, Lisa1, Autor
Barcikowski, Stephan1, Autor
Sanchez-Garcia, Elsa3, Autor           
Birkedal, Victoria4, Autor
Gatsogiannis, Christos5, Autor           
Ehrmann, Michael1, 6, Autor
Saccà, Barbara1, Autor
Affiliations:
1Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen, Universitätstrasse 2, Essen 45117, Germany, ou_persistent22              
2Department of Structural Biochemistry, Max-Planck-Institute of Molecular Physiology, Otto-Hahn-Straße 11, Dortmund 44227, Germany, ou_persistent22              
3Research Group Sánchez-García, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1950289              
4Department of Chemistry and Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, Aarhus 8000, Denmark, ou_persistent22              
5Abt. III: Strukturbiochemie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_2040307              
6School of Biosciences, Cardiff University, Cardiff CF10 3US, UK, ou_persistent22              

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 Zusammenfassung: The self-organizational properties of DNA have been used to realize synthetic hosts for protein encapsulation. However, current strategies of DNA–protein conjugation still limit true emulation of natural host–guest systems, whose formation relies on non-covalent bonds between geometrically matching interfaces. Here we report one of the largest DNA–protein complexes of semisynthetic origin held in place exclusively by spatially defined supramolecular interactions. Our approach is based on the decoration of the inner surface of a DNA origami hollow structure with multiple ligands converging to their corresponding binding sites on the protein surface with programmable symmetry and range-of-action. Our results demonstrate specific host–guest recognition in a 1:1 stoichiometry and selectivity for the guest whose size guarantees sufficient molecular diffusion preserving short intermolecular distances. DNA nanocontainers can be thus rationally designed to trap single guest molecules in their native form, mimicking natural strategies of molecular recognition and anticipating a new method of protein caging.

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Sprache(n): eng - English
 Datum: 2016-07-202017-01-032017-02-16
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1038/ncomms14472
 Art des Abschluß: -

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Titel: Nature Communications
  Kurztitel : Nat. Commun.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: London : Nature Publishing Group
Seiten: - Band / Heft: 8 Artikelnummer: 14472 (2017) Start- / Endseite: - Identifikator: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723