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  Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions

Sprengel, A., Lill, P., Stegemann, P., Bravo-Rodriguez, K., Schöneweiß, E.-C., Merdanovic, M., et al. (2017). Tailored protein encapsulation into a DNA host using geometrically organized supramolecular interactions. Nature Communications, 8: 14472 (2017). doi:10.1038/ncomms14472.

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 Creators:
Sprengel, Andreas1, Author
Lill, Pascal2, Author
Stegemann, Pierre1, Author
Bravo-Rodriguez, Kenny3, Author              
Schöneweiß, Elisa-C.1, Author
Merdanovic, Melisa1, Author
Gudnason, Daniel4, Author
Aznauryan, Mikayel4, Author
Gamrad, Lisa1, Author
Barcikowski, Stephan1, Author
Sanchez-Garcia, Elsa3, Author              
Birkedal, Victoria4, Author
Gatsogiannis, Christos5, Author              
Ehrmann, Michael1, 6, Author
Saccà, Barbara1, Author
Affiliations:
1Centre for Medical Biotechnology (ZMB), Centre for Nano Integration Duisburg-Essen, University of Duisburg-Essen, Universitätstrasse 2, Essen 45117, Germany, ou_persistent22              
2Department of Structural Biochemistry, Max-Planck-Institute of Molecular Physiology, Otto-Hahn-Straße 11, Dortmund 44227, Germany, ou_persistent22              
3Research Group Sánchez-García, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1950289              
4Department of Chemistry and Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, Aarhus 8000, Denmark, ou_persistent22              
5Abt. III: Strukturbiochemie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_2040307              
6School of Biosciences, Cardiff University, Cardiff CF10 3US, UK, ou_persistent22              

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 Abstract: The self-organizational properties of DNA have been used to realize synthetic hosts for protein encapsulation. However, current strategies of DNA–protein conjugation still limit true emulation of natural host–guest systems, whose formation relies on non-covalent bonds between geometrically matching interfaces. Here we report one of the largest DNA–protein complexes of semisynthetic origin held in place exclusively by spatially defined supramolecular interactions. Our approach is based on the decoration of the inner surface of a DNA origami hollow structure with multiple ligands converging to their corresponding binding sites on the protein surface with programmable symmetry and range-of-action. Our results demonstrate specific host–guest recognition in a 1:1 stoichiometry and selectivity for the guest whose size guarantees sufficient molecular diffusion preserving short intermolecular distances. DNA nanocontainers can be thus rationally designed to trap single guest molecules in their native form, mimicking natural strategies of molecular recognition and anticipating a new method of protein caging.

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Language(s): eng - English
 Dates: 2016-07-202017-01-032017-02-16
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/ncomms14472
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: 14472 (2017) Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723