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  Structural studies of Acidianus tailed spindle virus reveal a structural paradigm used in the assembly of spindle- shaped viruses

Hochstein, R., Bollschweiler, D., Dharmavaram, S., Lintner, N. G., Plitzko, J. M., Bruinsma, R., et al. (2018). Structural studies of Acidianus tailed spindle virus reveal a structural paradigm used in the assembly of spindle- shaped viruses. Proceedings of the National Academy of Sciences of the United States of America, 115(9), 2120-2125. doi:10.1073/pnas.1719180115.

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 Creators:
Hochstein, Rebecca1, Author
Bollschweiler, Daniel2, Author              
Dharmavaram, Sanjay1, Author
Lintner, Nathanael G.1, Author
Plitzko, Jürgen M.2, Author              
Bruinsma, Robijn1, Author
Engelhardt, Harald2, Author              
Young, Mark J.1, Author
Klug, William S.1, Author
Lawrence, C. Martin2, Author              
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: X-RAY CRYSTALLOGRAPHY; VOLTA PHASE PLATE; ELECTRON-MICROSCOPY; SULFOLOBUS; PROTEIN; TOMOGRAPHY; HOST; BACTERIOPHAGE-T4; VISUALIZATION; CONTRASTScience & Technology - Other Topics; Archaea; virus; lemon-shaped; ATSV; spindle-shaped;
 Abstract: The spindle-shaped virion morphology is common among archaeal viruses, where it is a defining characteristic of many viral families. However, structural heterogeneity intrinsic to spindle-shaped viruses has seriously hindered efforts to elucidate the molecular architecture of these lemon-shaped capsids. We have utilized a combination of cryo-electron microscopy and X-ray crystallography to study Acidianus tailed spindle virus (ATSV). These studies reveal the architectural principles that underlie assembly of a spindle-shaped virus. Cryo-electron tomography shows a smooth transition from the spindle-shaped capsid into the tubular-shaped tail and allows low-resolution structural modeling of individual virions. Remarkably, higher-dose 2D micrographs reveal a helical surface lattice in the spindle-shaped capsid. Consistent with this, crystallographic studies of the major capsid protein reveal a decorated four-helix bundle that packs within the crystal to form a four-start helical assembly with structural similarity to the tube-shaped tail structure of ATSV and other tailed, spindle-shaped viruses. Combined, this suggests that the spindle-shaped morphology of the ATSV capsid is formed by a multistart helical assembly with a smoothly varying radius and allows construction of a pseudoatomic model for the lemon-shaped capsid that extends into a tubular tail. The potential advantages that this novel architecture conveys to the life cycle of spindle-shaped viruses, including a role in DNA ejection, are discussed.

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Language(s): eng - English
 Dates: 2018-02-27
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000426152500063
DOI: 10.1073/pnas.1719180115
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proceedings of the National Academy of Sciences of the USA
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Abbreviation : PNAS
Source Genre: Journal
 Creator(s):
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 115 (9) Sequence Number: - Start / End Page: 2120 - 2125 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230