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  Optical Control of a Biological Reaction-Diffusion System

Glock, P., Broichhagen, J., Kretschmer, S., Blumhardt, P., Mücksch, J., Trauner, D., et al. (2018). Optical Control of a Biological Reaction-Diffusion System. Angewandte Chemie International Edition, 57(9), 2362-2366. doi:10.1002/anie.201712002.

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Genre: Journal Article

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 Creators:
Glock, Philipp1, Author              
Broichhagen, Johannes2, Author
Kretschmer, Simon1, Author              
Blumhardt, Philipp1, Author              
Mücksch, Jonas1, Author              
Trauner, Dirk2, Author
Schwille, P.1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              
2external, ou_persistent22              

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Free keywords: CELL-DIVISION; ESCHERICHIA-COLI; PATTERN-FORMATION; CHEMICAL-SYSTEM; MINE; PROTEINS; FEEDBACK; PEPTIDE; WAVESChemistry; chemical oscillators; optical control; photoswitches; pattern formation; synthetic biology;
 Abstract: Patterns formed by reaction and diffusion are the foundation for many phenomena in biology. However, the experimental study of reaction-diffusion (R-D) systems has so far been dominated by chemical oscillators, for which many tools are available. In this work, we developed a photoswitch for the Min system of Escherichia coli, a versatile biological in vitro R-D system consisting of the antagonistic proteins MinD and MinE. A MinE-derived peptide of 19 amino acids was covalently modified with a photoisomerizable crosslinker based on azobenzene to externally control peptide-mediated depletion of MinD from the membrane. In addition to providing an on-off switch for pattern formation, we achieve frequency-locked resonance with a precise 2D spatial memory, thus allowing new insights into Min protein action on the membrane. Taken together, we provide a tool to study phenomena in pattern formation using biological agents.

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Language(s): eng - English
 Dates: 2017-12-212018-02-23
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000425453300013
DOI: 10.1002/anie.201712002
 Degree: -

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Project name : Grant Numbers: GRK 2062, SFB 1032, SFB 152
Grant ID : -
Funding program : -
Funding organization : DFG

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Title: Angewandte Chemie International Edition
  Other : Angew. Chem., Int. Ed.
  Other : Angew. Chem. Int. Ed.
  Other : Angewandte Chemie, International Edition
Source Genre: Journal
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Affiliations:
Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 57 (9) Sequence Number: - Start / End Page: 2362 - 2366 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851