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  Relative levels of methylation in human growth hormone and chorionic somatomammotropin genes in expressing and non-expressing tissues

Hjelle, B. L., Phillips, J. A., & Seeburg, P. H. (1982). Relative levels of methylation in human growth hormone and chorionic somatomammotropin genes in expressing and non-expressing tissues. Nucleic Acids Research, 10(11), 3459-3474. doi:10.1093/nar/10.11.3459.

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Hjelle, B. L., Author
Phillips, John A., Author
Seeburg, Peter H.1, Author           
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1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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 Abstract: It has been shown that the extent of methylation of cytosine in vertebrate DNA is inversely correlated with gene expression. We studied cytosine methylation in and around the homologous human growth hormone (GH) and chorionic somatomammotropin (CS) genes to determine if these genes are undermethylated in DNA from tissues in which they are expressed (pituitary and placenta, respectively) compared to other tissues. Hpa II and Hha I (which cleave only unmethylated 5' CCGG 3' and 5' GCGC 3' respectively) and Msp I (which cleaves CCGG and CmeCGG) were used to digest DNA samples followed by gel electrophoresis, Southern transfer and hybridization with a GH cDNA probe. The extent of methylation of Hpa II and Hha I sites in the GH and CS genes was leukocyte much greater than pituitary greater than placenta = hydatidiform mole. Taken as a whole, our data support the hypothesis that undermethylation is a necessary but not sufficient condition for gene expression since placental and pituitary DNAs are less methylated than leukocyte DNA in this region. However, the correlation between gene expression and undermethylation is imperfect since (1) hydatiform mole DNA has a very similar methylation pattern compared to placental DNA even though moles make little or no CS and (2) the level of methylation of the GH gene compared to the CS gene does not vary in a tissue-specific manner.

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Language(s): eng - English
 Dates: 1982-03-291981-12-281982-05-131982-06-11
 Publication Status: Issued
 Pages: 16
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 Rev. Type: Peer
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Title: Nucleic Acids Research
  Other : Nucleic Acids Res.
Source Genre: Journal
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Pages: - Volume / Issue: 10 (11) Sequence Number: - Start / End Page: 3459 - 3474 Identifier: ISSN: 0301-5610
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000262810